A novel FexA variant from a canine Staphylococcus pseudintermedius isolate that does not confer florfenicol resistance

  1. Gómez-Sanz, E. 1
  2. Kadlec, K. 2
  3. Feßler, A.T. 2
  4. Zarazaga, M. 1
  5. Torres, C. 1
  6. Schwarz, S. 2
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Neustadt-Mariensee, Germany
Revista:
Antimicrobial Agents and Chemotherapy

ISSN: 0066-4804

Any de publicació: 2013

Volum: 57

Número: 11

Pàgines: 5763-5766

Tipus: Article

DOI: 10.1128/AAC.00948-13 SCOPUS: 2-s2.0-84885906775 WoS: WOS:000325764000081 GOOGLE SCHOLAR

Altres publicacions en: Antimicrobial Agents and Chemotherapy

Repositori institucional: lock_openAccés obert Editor

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Resum

Transposon Tn558 integrated in the chromosomal radC gene was detected for the first time in Staphylococus pseudintermedius. It carried a novel fexA variant (fexAv) that confers only chloramphenicol resistance. The exporter FexAv exhibited two amino acid substitutions, Gly33Ala and Ala37Val, both of which seem to be important for substrate recognition. Sitedirected mutagenesis that reverted the mutated base pairs to those present in the original fexA gene restored the chloramphenicol-plus-florfenicol resistance phenotype. Copyright © 2013, American Society for Microbiology. All Rights Reserved.