Characterization of the mechanisms of fluoroquinolone resistance in vancomycin-resistant enterococci of different origin

  1. Lopez, M. 1
  2. Tenorio, C. 1
  3. del Campo, R. 23
  4. Zarazaga, M. 1
  5. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    GRID grid.119021.a

  2. 2 Centro de Investigación Biomédica en Red sobre Epidemiología Y Salud Pública
    info

    Centro de Investigación Biomédica en Red sobre Epidemiología Y Salud Pública

    Madrid, España

    GRID grid.466571.7

  3. 3 Hospital Ramón y Cajal
    info

    Hospital Ramón y Cajal

    Madrid, España

    GRID grid.411347.4

Journal:
Journal of Chemotherapy

ISSN: 1120-009X

Year of publication: 2011

Volume: 23

Issue: 2

Pages: 87-91

Type: Article

Export: RIS

Metrics

Cited by

  • Scopus Cited by: 13 (12-06-2021)

Journal Citation Reports

  • Year 2011
  • Journal Impact Factor: 1.084
  • Best Quartile: Q4
  • Area: ONCOLOGY Quartile: Q4 Rank in area: 163/196 (Ranking edition: SCIE)
  • Area: PHARMACOLOGY & PHARMACY Quartile: Q4 Rank in area: 203/261 (Ranking edition: SCIE)

SCImago Journal Rank

  • Year 2011
  • SJR Journal Impact: 0.462
  • Best Quartile: Q2
  • Area: Pharmacology (medical) Quartile: Q2 Rank in area: 115/263
  • Area: Medicine (miscellaneous) Quartile: Q2 Rank in area: 1206/2921
  • Area: Infectious Diseases Quartile: Q3 Rank in area: 134/278
  • Area: Oncology Quartile: Q3 Rank in area: 166/329
  • Area: Pharmacology Quartile: Q3 Rank in area: 182/365

CiteScore

  • Year 2011
  • CiteScore: 2.2
  • Area: Pharmacology (medical) Percentile: 49
  • Area: Infectious Diseases Percentile: 49
  • Area: Oncology Percentile: 48
  • Area: Pharmacology Percentile: 44

Summary

The mutations in gyrA and parC genes were analyzed in 22 vancomycin-resistant enterococci of different origins and species, which had varying susceptibility to ciprofloxacin (minimum inhibitory concentration, MIC: 0.5->256 mg/L). All vanA or vanB2-containing strains with ciprofloxacin MIC of >32 mg/L presented amino acid changes in GyrA protein (S83I, S83Y, S83R or S83I-E87G) with/without changes in ParC protein (S80I or S80R or S80L). Strains with lower ciprofloxacin MlCs presented the GyrA and ParC wild type. One uanA-containing Enterococcus durans strain with a ciprofloxacin MIC of 64 mg/L presented the S83I and S80I changes in GyrA and ParC proteins, respectively. Two variB2 Enterococcus faecium strains were typed by multi-locus-sequence-typing and both were ascribed to the CC17 clonal complex with two sequence-types (ST78 and ST17-like). All seven vancomycin-resistant and ciprofloxacin-resistant E. faecium strains showed ampicillin resistance (MIC 32-256 mg/L), identifying the following amino acid changes in PBP5 protein: Q461K, V462K, H470Q, M485A, N496K, A499T, E525D, N546T, A558T, G582S, K632Q, P642L, E629V and P667S, together with a serine insertion at position 466'. The 12 Enterococcus gallinarum and Enterococcus cassetiflavus isolates included in the study exhibited an MIC for ciprofloxacin in the range 0.5-16 mg/L and no amino acid changes were identified in GyrA or ParC proteins. Specific mutations in gyrA and parC genes are associated with fluoroquinolone resistance in E. faecium and E. durans of different origins. © E.S.I.F.T. srl - Firenze.