DNA methylation increases throughout Arabidopsis development

  1. Ruiz-García, L. 1
  2. Cervera, M.T. 12
  3. Martínez-Zapater, J.M. 1
  1. 1 Universidad Autónoma de Madrid
    info

    Universidad Autónoma de Madrid

    Madrid, España

    ROR https://ror.org/01cby8j38

  2. 2 Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
    info

    Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria

    Madrid, España

    ROR https://ror.org/011q66e29

Revista:
Planta

ISSN: 0032-0935

Año de publicación: 2005

Volumen: 222

Número: 2

Páginas: 301-306

Tipo: Artículo

DOI: 10.1007/S00425-005-1524-6 PMID: 15968513 SCOPUS: 2-s2.0-26444479664 GOOGLE SCHOLAR

Otras publicaciones en: Planta

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

We used amplified fragment length polymorphisms (AFLP) to analyze the stability of DNA methylation throughout Arabidopsis development. AFLP can detect genome-wide changes in cytosine methylation produced by DNA demethylation agents, such as 5-azacytidine, or specific mutations at the DDM1 locus. In both cases, cytosine demethylation is associated with a general increase in the presence of amplified fragments. Using this approach, we followed DNA methylation at methylation sensitive restriction sites throughout Arabidopsis development. The results show a progressive DNA methylation trend from cotyledons to vegetative organs to reproductive organs. © Springer-Verlag 2005.