DNA methylation increases throughout Arabidopsis development
- Ruiz-García, L. 1
- Cervera, M.T. 12
- Martínez-Zapater, J.M. 1
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1
Universidad Autónoma de Madrid
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2
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
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Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
Madrid, España
ISSN: 0032-0935
Año de publicación: 2005
Volumen: 222
Número: 2
Páginas: 301-306
Tipo: Artículo
Otras publicaciones en: Planta
Resumen
We used amplified fragment length polymorphisms (AFLP) to analyze the stability of DNA methylation throughout Arabidopsis development. AFLP can detect genome-wide changes in cytosine methylation produced by DNA demethylation agents, such as 5-azacytidine, or specific mutations at the DDM1 locus. In both cases, cytosine demethylation is associated with a general increase in the presence of amplified fragments. Using this approach, we followed DNA methylation at methylation sensitive restriction sites throughout Arabidopsis development. The results show a progressive DNA methylation trend from cotyledons to vegetative organs to reproductive organs. © Springer-Verlag 2005.