Characterization of Vitis vinifera L. somatic variants exhibiting abnormal flower development patterns

  1. Chatelet, P. 3
  2. Laucou, V. 3
  3. Fernandez, L. 1
  4. Sreekantan, L. 2
  5. Lacombe, T. 3
  6. Martinez-Zapater, J.M. 1
  7. Thomas, M.R. 2
  8. Torregrosa, L. 3
  1. 1 Centro Nacional de Biotecnología
    info

    Centro Nacional de Biotecnología

    Madrid, España

    ROR https://ror.org/015w4v032

  2. 2 Commonwealth Scientific and Industrial Research Organisation
    info

    Commonwealth Scientific and Industrial Research Organisation

    Canberra, Australia

    ROR https://ror.org/03qn8fb07

  3. 3 Institut National de la Recherche Agronomique
    info

    Institut National de la Recherche Agronomique

    París, Francia

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Revista:
Journal of Experimental Botany

ISSN: 0022-0957

Año de publicación: 2007

Volumen: 58

Número: 15-16

Páginas: 4107-4118

Tipo: Artículo

DOI: 10.1093/JXB/ERM269 PMID: 18042806 SCOPUS: 2-s2.0-40049101979 GOOGLE SCHOLAR lock_openDigital CSIC editor

Otras publicaciones en: Journal of Experimental Botany

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

Mutants have proven to be a key resource for functional genomic studies in model annual plant species. In perennial plant species where mutants are difficult to generate and to screen, spontaneous somatic variants represent a unique resource to understand the genetic control of complex developmental patterns. The morphological and histological characterization of six Vitis vinifera L. somatic variants that display four different abnormal phenotypes of flower development are described here. A phenotype of reiterated reproductive meristems (RRM), with both flower and petal reiteration, was observed in a somatic variant of the cultivar Carignan. An abnormal development of reproductive organs was displayed by the unfused carpels (UFC) somatic variant of cv. Bouchalès, while a somatic variant of cv. Mourvèdre named carpel-less (CLS) developed abnormal ovules in the absence of carpels. Finally, three independent somatic variants in cvs Gamay, Morrastel, and Pinot displayed a phenotype of multiple perianth whorls (MPW). Gene expression studies showed that the expression profiles of VvMADS-box 1, 2, and 3 (putative orthologues of Arabidopsis flowering genes AG, SEP, and AGL13), were altered during grapevine flower development in the somatic variants, whereas the corresponding original cultivars displayed similar VvMADS-box gene expression profiles. Phenotypic and molecular characterization of these variants allowed the development of hypotheses on genetic functions that might be altered in most of the variants in light of the current ABCDE flower model. © 2007 The Author(s).