Identification of genes specifically expressed in cauliflower reproductive meristems. Molecular characterization of BoREM1

  1. Franco-Zorrilla, J.M. 1
  2. Fernández-Calvín, B. 2
  3. Madueño, F. 4
  4. Cruzalvarez, M. 3
  5. Salinas, J. 2
  6. Martínez-Zapater, J.M. 2
  1. 1 Centro Nacional de Biotecnología
    info

    Centro Nacional de Biotecnología

    Madrid, España

    ROR https://ror.org/015w4v032

  2. 2 Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
    info

    Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria

    Madrid, España

    ROR https://ror.org/011q66e29

  3. 3 Salem State University
    info

    Salem State University

    Salem, Estados Unidos

    ROR https://ror.org/023qmza96

  4. 4 Instituto de Biología Molecular y Celular de Plantas Primo Yufera
    info

    Instituto de Biología Molecular y Celular de Plantas Primo Yufera

    Valencia, España

    ROR https://ror.org/04zdays56

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Revista:
Plant Molecular Biology

ISSN: 0167-4412

Any de publicació: 1999

Volum: 39

Número: 3

Pàgines: 427-436

Tipus: Article

DOI: 10.1023/A:1006130629100 PMID: 10092172 SCOPUS: 2-s2.0-0345390087 GOOGLE SCHOLAR

Altres publicacions en: Plant Molecular Biology

Repositori institucional: lock_openAccés obert Editor

Resum

Using the meristems of the cauliflower curd as a source of tissue and a series of subtractive hybridizations and amplification reactions, we have constructed a cDNA library highly enriched in cDNAs expressed in reproductive meristems. The analysis of a sample of 250 clones from this library identified 22 cDNA clones corresponding to genes specifically expressed in these cauliflower meristems. Apart from two clones that corresponded to APETALA1, and two other ones showing similarity to different aminoacyl-tRNA synthetases, the remaining clones showed no similarity to any sequence in the databases and may correspond to novel genes. One of these clones, BoREM1, was further characterized and found to correspond to a gene encoding a protein with features of regulatory proteins that follows a expression pattern very similar to the LEAFY transcripts.