Genetic relationships among biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae) based on AFLP analysis

  1. Cervera, M.T. 13
  2. Cabezas, J.A. 13
  3. Simon, B. 2
  4. Martinez-Zapater, J.M. 13
  5. Beitia, F. 3
  6. Cenis, J.L. 2
  1. 1 Centro Nacional de Biotecnología
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    Centro Nacional de Biotecnología

    Madrid, España

    ROR https://ror.org/015w4v032

  2. 2 Instituto Murciano de Investigacion y Desarrollo Agrario y Alimentario
    info

    Instituto Murciano de Investigacion y Desarrollo Agrario y Alimentario

    La Alberca de Záncara, España

  3. 3 Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
    info

    Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria

    Madrid, España

    ROR https://ror.org/011q66e29

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Revista:
Bulletin of Entomological Research

ISSN: 0007-4853

Año de publicación: 2000

Volumen: 90

Número: 5

Páginas: 391-396

Tipo: Artículo

PMID: 11082556 SCOPUS: 2-s2.0-0033656852 GOOGLE SCHOLAR lock_openDigital CSIC editor

Otras publicaciones en: Bulletin of Entomological Research

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

Genetic similarities between 13 samples belonging to nine reference biotypes and two field populations of Bemisia tabaci (Gennadius), one field population of B. medinae Gomez-Menor and another of B. afer Priesner and Hosny, were evaluated using amplified fragment length polymorphism (AFLP) markers. The results indicate that B. tabaci biotypes can be grouped together with a minimum similarity coefficient of 0.32 and separated from the two other species with a similarity coefficient of 0.07. Bemisia tabaci biotypes were grouped in four clusters which comprised: (i) Near East and Indian subcontinent biotypes; (ii) B and Q biotypes plus a Nigerian population from cowpea; (iii) New World A biotype; and (iv) S biotype and a Nigerian population from cassava. These results were consistent with a previous grouping of biotypes based on RAPD-PCR analysis. The AFLP assay allowed the scoring of a total of 354 polymorphic bands in two reaction events with the use of two primer combinations.