In situ random primer extension of metaphase chromosomes

  1. Garcia de la Vega, C. 1
  2. Martinez Zapater, J.M. 1
  3. Lopez-Fernandez, C. 1
  4. Goyanes, Vicente . 1
  5. Mezzanotte, Roberto . 1
  6. Gosalvez, J. 1
  1. 1 Universidad Autónoma de Madrid
    info

    Universidad Autónoma de Madrid

    Madrid, España

    ROR https://ror.org/01cby8j38

Revista:
Cytogenetics and Cell Genetics

ISSN: 0301-0171

Año de publicación: 1990

Volumen: 53

Número: 4

Páginas: 211-212

Tipo: Artículo

DOI: 10.1159/000132932 PMID: 2209088 SCOPUS: 2-s2.0-0025092317 WoS: WOS:A1990ED25500007 GOOGLE SCHOLAR

Otras publicaciones en: Cytogenetics and Cell Genetics

Resumen

We have developed a technique of random primer extension of fixed chromosomes that is applicable to both mouse and man. Human chromosomes are not homogeneously labeled with this technique; those regions corresponding to R-bands appear to be more sensitive than those identified as G-bands, whereas centromeric regions are not labeled. These results not only corroborate specific structural differences between distinct regions of mammalian genomes but also open up the possibility of assays with specific primers to test whether primer extension is useful for the identification of genes and families of sequences on chromosomes.