Transcriptome and metabolome reprogramming in Vitis vinifera cv. Trincadeira berries upon infection with Botrytis cinerea

  1. Agudelo-Romero, P. 1
  2. Erban, A. 3
  3. Rego, C. 1
  4. Carbonell-Bejerano, P. 2
  5. Nascimento, T. 1
  6. Sousa, Lisete. 1
  7. Martínez-Zapater, J.M. 2
  8. Kopka, J. 3
  9. Fortes, A.M. 1
  1. 1 Universidade de Lisboa
    info

    Universidade de Lisboa

    Lisboa, Portugal

    ROR https://ror.org/01c27hj86

  2. 2 Instituto de Ciencias de la Vid y del Vino
    info

    Instituto de Ciencias de la Vid y del Vino

    Logroño, España

    ROR https://ror.org/01rm2sw78

  3. 3 Max Planck Institute for Molecular Plant Physiology
    info

    Max Planck Institute for Molecular Plant Physiology

    Potsdam, Alemania

    ROR https://ror.org/01fbde567

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Revista:
Journal of Experimental Botany

ISSN: 0022-0957

Año de publicación: 2015

Volumen: 66

Número: 7

Páginas: 1769-1785

Tipo: Artículo

DOI: 10.1093/JXB/ERU517 SCOPUS: 2-s2.0-84928905868 WoS: WOS:000353894100007 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Experimental Botany

Resumen

Vitis vinifera berries are sensitive towards infection by the necrotrophic pathogen Botrytis cinerea, leading to important economic losses worldwide. The combined analysis of the transcriptome and metabolome associated with fungal infection has not been performed previously in grapes or in another fleshy fruit. In an attempt to identify the molecular and metabolic mechanisms associated with the infection, peppercorn-sized fruits were infected in the field. Green and veraison berries were collected following infection for microarray analysis complemented with metabolic profiling of primary and other soluble metabolites and of volatile emissions. The results provided evidence of a reprogramming of carbohydrate and lipid metabolisms towards increased synthesis of secondary metabolites involved in plant defence, such as trans-resveratrol and gallic acid. This response was already activated in infected green berries with the putative involvement of jasmonic acid, ethylene, polyamines, and auxins, whereas salicylic acid did not seem to be involved. Genes encoding WRKY transcription factors, pathogenesis-related proteins, glutathione S-transferase, stilbene synthase, and phenylalanine ammonia-lyase were upregulated in infected berries. However, salicylic acid signalling was activated in healthy ripening berries along with the expression of proteins of the NBS-LRR superfamily and protein kinases, suggesting that the pathogen is able to shut down defences existing in healthy ripening berries. Furthermore, this study provided metabolic biomarkers of infection such as azelaic acid, a substance known to prime plant defence responses, arabitol, ribitol, 4-amino butanoic acid, 1-O-methyl- glucopyranoside, and several fatty acids that alone or in combination can be used to monitor Botrytis infection early in the vineyard. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.