B-Chromosomes and E-1 isozyme activity in mosaic bulbs of Scilla autumnalis (Liliaceae)

  1. Oliver, J.L. 1
  2. Posse, F. 1
  3. Martinez-Zapater, J.M. 1
  4. Enriquez, A.M. 1
  5. Ruiz-Rejón, M. 2
  1. 1 Universidad Autónoma de Madrid
    info

    Universidad Autónoma de Madrid

    Madrid, España

    ROR https://ror.org/01cby8j38

  2. 2 Universidad de Granada
    info

    Universidad de Granada

    Granada, España

    ROR https://ror.org/04njjy449

Revista:
Chromosoma

ISSN: 0009-5915

Año de publicación: 1982

Volumen: 85

Número: 3

Páginas: 399-403

Tipo: Artículo

DOI: 10.1007/BF00330362 SCOPUS: 2-s2.0-0019940621 GOOGLE SCHOLAR

Otras publicaciones en: Chromosoma

Resumen

The electrophoretic patterns of esterase (E-1), alcohol dehydrogenase (ADH), and glutamate oxaloacetate transaminase (GOT) isozymes were studied in two Spanish populations of the lily Scilla autumnalis with B-chromosome carrying individuals. The E-1 isozyme activity appears only in those individuals with B-chromosomes. None of the bulbs free of B's show it. Five bulbs, mosaic for B-content, were identified. Electrophoretic analysis shows that these bulbs are characterised by mosaicism for E-1 isozyme activity. An analysis of individual roots by both electrophoretic and cytological methods shows that tissue mosaicism for B-content correlates with tissue mosaicism for E-1 isozyme activity. The electrophoretic analysis of different roots from bulbs heterozygous for the Est-1 locus indicates that the structural gene for E-1 is not located on the B-chromosome itself. Rather there is a derepressor effect of Bs on E-1 isozyme activity. Since ADH and GOT patterns are unaffected by the presence of B-chromosomes it is clear that they do not exhibit a generalised derepressor effect. © 1982 Springer-Verlag.