Molecular characterization of staphylococcus aureus from nasal samples of healthy farm animals and pets in Tunisia

  1. Gharsa, H. 3
  2. Slama, K.B. 3
  3. Gómez-Sanz, E. 12
  4. Lozano, C. 1
  5. Zarazaga, M. 1
  6. Messadi, L. 4
  7. Boudabous, A. 3
  8. Torres, C. 2
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Zurich University of Applied Sciences
    info

    Zurich University of Applied Sciences

    Winterthur, Suiza

    ROR https://ror.org/05pmsvm27

  3. 3 Université de Tunis El Manar
    info

    Université de Tunis El Manar

    Túnez, Túnez

    ROR https://ror.org/029cgt552

  4. 4 Manouba University
    info

    Manouba University

    Manouba, Túnez

    ROR https://ror.org/0503ejf32

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Revista:
Vector-Borne and Zoonotic Diseases

ISSN: 1530-3667

Año de publicación: 2015

Volumen: 15

Número: 2

Páginas: 109-115

Tipo: Artículo

DOI: 10.1089/VBZ.2014.1655 SCOPUS: 2-s2.0-84923464406 WoS: WOS:000349682500004 GOOGLE SCHOLAR

Otras publicaciones en: Vector-Borne and Zoonotic Diseases

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2013/00001/001

Resumen

A total of 261 healthy farm and pet animals (75 cattle, 52 goats, 100 dogs, and 34 cats) from different regions of Tunisia were screened for Staphylococcus aureus nasal carriage. Molecular typing of isolates (by spa-and multilocus sequence-typing) was performed, and their antimicrobial resistance and virulence genotypes were determined by PCR and sequencing. S. aureus isolates were detected in 17 of 261 tested samples (6.5%). All S. aureus isolates recovered were methicillin-susceptible (MSSA), and one isolate/sample was further studied. Eight different spa types were detected (t189, t279, t582, t701, t1166, t1268, t1534, and t1773), and eight different sequence types were identified (ST6, ST15, ST45, ST133, ST188, ST700 [clonal complex CC130], ST2057, and a new ST2121). MSSA from pets (six isolates) showed resistance to (number of isolates, resistance gene): penicillin (six, blaZ), tetracycline (one, tet[M]), erythromycin one, erm[A]), streptomycin (one, ant[6]-Ia), and ciprofloxacin (one). All isolates from farm animals showed susceptibility to the tested antimicrobials, except for two penicillin-resistant isolates. Five S. aureus isolates from goats and cats harbored the lukF/lukSPV genes, encoding the Panton-Valentine leukocidin, and six isolates from goats harbored the tst virulence gene. In addition, diverse combinations of enterotoxin genes were detected, including two variants of the egc cluster. Goats and cats could represent a reservoir of important toxin genes, with potential implications in animal and human health. © 2015 Mary Ann Liebert, Inc.