Clonal Dynamics of Nasal Staphylococcus aureus and Staphylococcus pseudintermedius in Dog-Owning Household Members. Detection of MSSA ST398

  1. Gómez-Sanz, E. 1
  2. Torres, C. 1
  3. Ceballos, S. 1
  4. Lozano, C. 1
  5. Zarazaga, M. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    GRID grid.119021.a

Journal:
PLoS ONE

ISSN: 1932-6203

Year of publication: 2013

Volume: 8

Issue: 7

Type: Article

Export: RIS
DOI: 10.1371/journal.pone.0069337 SCOPUS: 2-s2.0-84879931517 WoS: 000321736900154 GOOGLE SCHOLAR lock_openOpen access editor
Institutional repository: lock_openOpen access editor

Metrics

Cited by

  • Scopus Cited by: 22 (12-06-2021)

Journal Citation Reports

  • Year 2013
  • Journal Impact Factor: 3.534
  • Best Quartile: Q1
  • Area: MULTIDISCIPLINARY SCIENCES Quartile: Q1 Rank in area: 8/55 (Ranking edition: SCIE)

SCImago Journal Rank

  • Year 2013
  • SJR Journal Impact: 1.772
  • Best Quartile:
  • Area: Multidisciplinary Quartile: - Rank in area: 5/129

CiteScore

  • Year 2013
  • CiteScore of the Journal : 4.4
  • Area: Agricultural and Biological Sciences (all) Percentile: 87
  • Area: Biochemistry, Genetics and Molecular Biology (all) Percentile: 78
  • Area: Medicine (all) Percentile: 69

Abstract

The objective of this study was to investigate the dynamics of nasal carriage by Staphylococcus aureus (SA) and Staphylococcus pseudintermedius (SP) among healthy dog-owning household members involved in 7 previous index cases of suspected anthropozoonotic (n = 4) and zoonotic (n = 3) interspecies transmission [4 direct cases, identical SA (n = 3) or SP (n = 1) in owner and dog; three indirect, SP in owner (n = 2) or SA in dog (n = 1)]. Co-carriage with methicillin-resistant coagulase-negative staphylococci (MRCoNS) was also evaluated. Sixteen owners and 10 dogs were sampled once every three months for one year. In total, 50 SA and 31 SP were analysed by MLST, and SA also by spa typing. All isolates were subjected to ApaI/SmaI-PFGE and antimicrobial resistance and virulence profiles were determined. All index owners were persistent SA carriers in all direct-anthropozoonotic transmission cases, while only one dog was persistent SA carrier. Owner and dog exhibited a persistent SP carriage status in the direct-zoonotic transmission case. SP was maintained in the index human over time in one indirect-zoonotic transmission case. Only one SP was methicillin-resistant. SA belonged to genetic backgrounds of MRSA pandemic clones: CC45, CC121, CC30, CC5 and CC398. Three individuals carried a MSSA t1451-ST398 clone with the erm(T)-cadD/cadX resistance genes. SA or SP were persistently detected in the nasal cavity of 7 (43.8%) and 2 (12.5%) owners, and in one and 2 dogs, respectively. SA was recovered as the single species in 10 owners and in one dog; SP in 3 owners and 4 dogs; and both bacterial species in one owner and 4 dogs. Co-carriage of SA or SP with MRCoNS isolates was common (30.7%). This is the first study on the dynamics of nasal carriage of SA and SP in healthy pet-owning household members. Dog-contact may play a role in the staphylococcal species distribution of in-contact individuals. © 2013 Gómez-Sanz et al.