Prevalence of extended-spectrum beta-lactamase-producing Escherichia coli isoaltes in faecal samples of broilers

  1. Costa, D. 15
  2. Vinué, L. 1
  3. Poeta, P. 25
  4. Coelho, A.C. 5
  5. Matos, M. 4
  6. Sáenz, Y. 13
  7. Somalo, S. 1
  8. Zarazaga, M. 13
  9. Rodrigues, J. 25
  10. Torres, C. 13
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Centre of Studies of Animal and Veterinary Sciences, Vila Real, Portugal
  3. 3 Centro de Investigación Biomédica de La Rioja
    info

    Centro de Investigación Biomédica de La Rioja

    Logroño, España

    ROR https://ror.org/03vfjzd38

  4. 4 Departamento de Genética e Biotecnología, Instituto de Biotecnología e Bioengenharia, Vila Real, Portugal
  5. 5 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

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Revista:
Veterinary Microbiology

ISSN: 0378-1135

Año de publicación: 2009

Volumen: 138

Número: 3

Páginas: 339-344

Tipo: Artículo

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DOI: 10.1016/J.VETMIC.2009.03.029 SCOPUS: 2-s2.0-69349090273 WoS: WOS:000270246800017 GOOGLE SCHOLAR

Otras publicaciones en: Veterinary Microbiology

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Resumen

Seventy-six faecal samples were obtained from broilers at slaughterhouse level in Portugal. Samples were inoculated on cefotaxime-supplemented Levine agar plates. Cefotaxime-resistant Escherichia coli isolates were recovered from 32 samples (42.1%), obtaining a total of 34 E. coli isolates (one or two isolates per sample). Susceptibility to 16 antibiotics was studied by disk diffusion method, and 85% of the isolates presented a phenotype of multi-resistance that included antimicrobial agents of at least four different families. Extended-spectrum-beta-lactamases (ESBL) of the TEM and CTX-M groups were detected in 31 ESBL-positive E. coli isolates. Twenty-six isolates harboured the bla TEM-52 gene and two of them also harboured bla TEM-1b. The bla CTX-M-14 gene was identified in three isolates (in association with bla TEM-1b in one of them), and bla CTX-M-32 was demonstrated in two additional isolates. Three of the 34 cefotaxime-resistant isolates (9%) did not produce ESBLs, and two of them presented mutations at positions -42 (C → T), -18 (G → A), -1 (C → T), and +58(C → T) of the promoter/attenuator region of ampC gene. tet(A) and/or tet(B) genes were detected in all 34 tetracycline-resistant isolates, aadA in all 26 streptomycin-resistant isolates; cmlA in 3 of 6 chloramphenicol-resistant isolates, and aac(3)-II or aac(3)-I + aac(3)-IV genes in all 4 gentamicin-resistant isolates. Different combinations of sul1, sul2 and sul3 genes were demonstrated among the 22 trimethoprim-sulfamethoxazole-resistant isolates. Amino acid changes in GyrA and ParC proteins were identified in all 18 ciprofloxacin-resistant isolates. The results of this study indicate that the intestinal tract of healthy poultry is a reservoir of ESBL-positive E. coli isolates. © 2009 Elsevier B.V. All rights reserved.