Genetic characterisation of CTX-M-15-producing Klebsiella pneumoniae and Escherichia coli strains isolated from stem cell transplant patients in Tunisia

  1. Abbassi, M.S. 1
  2. Torres, C. 2
  3. Achour, W. 1
  4. Vinué, L. 2
  5. Sáenz, Y. 2
  6. Costa, D. 2
  7. Bouchami, O. 1
  8. Ben Hassen, A. 1
  1. 1 Laboratory of the National Bone Marrow Transplantation Centre, Bab Saadoun, Tunis, Tunisia
  2. 2 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

Revista:
International Journal of Antimicrobial Agents

ISSN: 0924-8579

Año de publicación: 2008

Volumen: 32

Número: 4

Páginas: 308-314

Tipo: Artículo

DOI: 10.1016/J.IJANTIMICAG.2008.04.009 PMID: 18620848 SCOPUS: 2-s2.0-50849096678 WoS: WOS:000258992300004 GOOGLE SCHOLAR

Otras publicaciones en: International Journal of Antimicrobial Agents

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Resumen

Characterisation of extended-spectrum β-lactamase (ESBL) genes and their genetic environments as well as the presence of integrons were analysed in nine Klebsiella pneumoniae and two Escherichia coli ESBL-positive isolates recovered in the Centre of Bone Marrow Transplantation of Tunisia. All strains harboured the blaCTX-M-15 gene and presented minimum inhibitory concentrations for cefotaxime and ceftazidime of 256-1024 mg L-1 and 16-512 mg L-1, respectively, and eight of them showed different pulsed-field gel electrophoresis patterns. The blaOXA-1 and blaTEM-1 genes were detected in eight and ten strains, respectively. In addition, blaSHV-1, blaSHV-11 and blaSHV-27 were found in six, one and one K. pneumoniae strains, respectively. The new variant blaSHV-103 was characterised in one K. pneumoniae strain. The intI1 gene was detected in eight K. pneumoniae strains and the dfrA5 + ereA2 and aadA gene cassettes were found in one and five strains, respectively. All strains harboured a 70 kb plasmid, and its transference in addition to blaCTX-M-15, blaTEM-1b and blaOXA-1 genes was demonstrated from three K. pneumoniae to E. coli. ISEcp1 and orf477 were located upstream and downstream, respectively, of the blaCTX-M-15 gene in 10 strains. The occurrence of the blaCTX-M-15 gene in unrelated strains might have originated from the dissemination of mobile genetic elements in which ISEcp1 may have played an important role. © 2008 Elsevier B.V. and the International Society of Chemotherapy.