Escherichia coli of poultry food origin as reservoir of sulphonamide resistance genes and integrons.

  1. Soufi, L. 13
  2. Sáenz, Y. 2
  3. Vinué, L. 1
  4. Abbassi, M.S. 3
  5. Ruiz, E. 1
  6. Zarazaga, M. 1
  7. Ben Hassen, A. 4
  8. Hammami, S. 35
  9. Torres, C. 12
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Centro de Investigación Biomédica de La Rioja
    info

    Centro de Investigación Biomédica de La Rioja

    Logroño, España

    ROR https://ror.org/03vfjzd38

  3. 3 Institute of Veterinary Research of Tunisia, Tunis 1006, Tunisia
  4. 4 Laboratory of the National Bone Marrow Transplantation Centre, Bab Saadoun, Tunis, Tunisia
  5. 5 National Center for Zoosanitary Vigilance, Tunis, Tunisia
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Revista:
International Journal of Food Microbiology

ISSN: 0168-1605

Año de publicación: 2011

Volumen: 144

Número: 3

Páginas: 497-502

Tipo: Artículo

DOI: 10.1016/J.IJFOODMICRO.2010.11.008 PMID: 21131082 SCOPUS: 2-s2.0-78650031706 WoS: WOS:000287227000024 GOOGLE SCHOLAR

Otras publicaciones en: International Journal of Food Microbiology

Resumen

The antimicrobial resistance phenotype and genotype, the flanking regions of sulphonamide resistance genes and the integrons were analyzed in 166 Escherichia coli isolates recovered from poultry meat in Tunisia. High percentages of resistance were detected to ampicillin, streptomycin, nalidixic acid, sulphonamide and tetracycline (66-95%), and lower percentages to gentamicin, amoxicillin-clavulanic acid and cefoxitin (1-4%). The bla TEM, tet(A)/tet(B), aph(3')-Ia, aac(6')-Ib-cr, aac(3)-II and cmlA genes were identified in 92, 82, 29, 2, 2 and 7 isolates, respectively. Class 1 and/or class 2 integrons were detected in 52% of E. coli isolates and five different gene cassette arrangements were identified in the variable regions of class 1 integrons, which included antimicrobial resistance determinants. Sixty-eight isolates contained the sul1 gene and 37 of them presented this gene into a class 1 integron structure. The sul3 gene was detected associated with non-classic class 1 integrons in 4 out of 46 sul3-positive isolates. The sul2 gene was detected in 66 isolates, 51 of them were linked to strA/B genes in seven different genetic structures. Seventy-three-per-cent of integron-positive isolates presented resistance to at least five different antimicrobial families versus 38.7% of integron-negative isolates. Our study highlights the role of commensal E. coli isolates from poultry meat as an important reservoir for sulphonamide resistance genes and integrons carrying antimicrobial resistance genes. © 2010 Elsevier B.V.