IncI1 plasmids carrying blaCTX-M-1or blaCMY-2genes in Escherichia coli from healthy humans and animals in Tunisia

  1. Ben Sallem, R. 3
  2. Ben Slama, K. 3
  3. Rojo-Bezares, B. 2
  4. Porres-Osante, N. 12
  5. Jouini, A. 4
  6. Klibi, N. 3
  7. Boudabous, A. 3
  8. Sáenz, Y. 2
  9. Torres, C. 12
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Centro de Investigación Biomédica de La Rioja
    info

    Centro de Investigación Biomédica de La Rioja

    Logroño, España

    ROR https://ror.org/03vfjzd38

  3. 3 Université de Tunis El Manar
    info

    Université de Tunis El Manar

    Túnez, Túnez

    ROR https://ror.org/029cgt552

  4. 4 Institut Pasteur de Tunis
    info

    Institut Pasteur de Tunis

    Túnez, Túnez

    ROR https://ror.org/04pwyer06

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Revista:
Microbial Drug Resistance

ISSN: 1076-6294

Año de publicación: 2014

Volumen: 20

Número: 5

Páginas: 495-500

Tipo: Artículo

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DOI: 10.1089/MDR.2013.0224 SCOPUS: 2-s2.0-84907450774 WoS: WOS:000342303200018 GOOGLE SCHOLAR

Otras publicaciones en: Microbial Drug Resistance

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Resistencia bacteriana a antimicrobianos en ambiente y fauna salvaje. Mecanismos de resistencia y líneas genéticas emergentes e implicaciones en salud humana

2013/00001/001

Resumen

The objective was to determine the location of blaCTX-M-1and blaCMY-2genes in 33 Escherichia coli isolates previously obtained from healthy humans, pets, and food-producing animals in Tunisia, and to characterize the genetic lineages of isolates. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE)-XbaI and multilocus sequence typing (MLST). Plasmids were analyzed by S1-PFGE, polymerase chain reaction-based replicon typing, and plasmid MLST. Conjugation experiments were performed. The blaCTX-M-1and blaCMY-2genes were studied by I-Ceu1-PFGE and S1-PFGE, and subsequent hybridization with specific probes. Eighteen different sequence types (STs) were identified among the 30 CTX-M-1-producing isolates, 5 of them being detected in 17 isolates (ST/phylogroup): ST57/D, ST155/B1, ST58/B1, ST10/A, and ST398/A. Most of the blaCTX-M-1-positive isolates had different PFGE profiles, with the exception of four human and pet isolates of lineage ST57 with related PFGE profiles (>80% identity). Three CMY-2-producing isolates were typed as ST58/B1, ST117/D, and ST3632/B2. The IncI1 replicon was detected in all the 33 E. coli studied isolates, in many cases in combination with other replicons: IncF, IncX, IncK, IncR, IncY, colE, or IncN. The blaCTX-M-1gene was transferred by conjugation in 22 of the 30 positive strains and was located into IncI1 plasmids (ST3-CC3); the blaCMY-2gene was located into a conjugative IncI1 plasmid (ST12) of 97 kb in one strain. One blaCTX-M-1-positive strain carried the qnrB19 gene in a 33 kb IncX plasmid. Diverse genetic lineages are detected in extended-spectrum beta-lactamase- and AmpC beta-lactamase-producing E. coli from different origins. The blaCTX-M-1and blaCMY-2genes were associated with conjugative IncI1 (ST3 and ST12, respectively) plasmids in E. coli strains from human and animal origin.