The Unusual Nucleotide Recognition Properties of the Resistance Enzyme ANT(4 '): Inorganic Tri/Polyphosphate as a Substrate for Aminoglycoside Inactivation

  1. Revuelta, J. 1
  2. Corzana, F. 2
  3. Bastida, A. 1
  4. Asensio, J.L. 1
  1. 1 Instituto de Química Orgánica General
    info

    Instituto de Química Orgánica General

    Madrid, España

    ROR https://ror.org/05e0q7s59

  2. 2 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

Revista:
Chemistry - A European Journal

ISSN: 0947-6539

Año de publicación: 2010

Volumen: 16

Número: 29

Páginas: 8635-8640

Tipo: Artículo

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DOI: 10.1002/CHEM.201000641 PMID: 20512827 SCOPUS: 2-s2.0-77955144385 WoS: WOS:000281060800011 GOOGLE SCHOLAR

Otras publicaciones en: Chemistry - A European Journal

Resumen

A pocket of resistance: We have analysed the molecular determinants for nucleotide recognition by the resistance enzyme ANT(4′). Our results demonstrate that its binding epitope is restricted to the inorganic triphosphate fragment. Strikingly, this, together with longer polyphosphate oligomers, can be employed as cosubstrates in aminoglycoside inactivation (see figure), implying a change in the normal activity of the enzyme. These results have implications in the design of specific enzymatic inhibitors. (Figure Presented) © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.