Nasal carriage of Staphylococcus aureus in healthy humans with different level of contact with animals in Tunisia: genetic lineages, methicillin-resistance and virulence factors.

  1. Ben Slama, K. 2
  2. Gharsa, H. 2
  3. Klibi, N. 2
  4. Jouini, A. 2
  5. Lozano, C. 1
  6. Gómez-Sanz, E. 1
  7. Zarazaga, M. 1
  8. Boudabous, A. 2
  9. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Université de Tunis El Manar
    info

    Université de Tunis El Manar

    Túnez, Túnez

    ROR https://ror.org/029cgt552

Revista:
European Journal of Clinical Microbiology and Infectious Diseases

ISSN: 0934-9723

Año de publicación: 2011

Volumen: 30

Número: 4

Páginas: 499-508

Tipo: Artículo

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DOI: 10.1007/S10096-010-1109-6 PMID: 21076928 SCOPUS: 2-s2.0-79956044872 WoS: WOS:000288219000004 GOOGLE SCHOLAR

Otras publicaciones en: European Journal of Clinical Microbiology and Infectious Diseases

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Resumen

Nasal swabs of 423 healthy humans who showed different levels of contact with animals (frequent, 168; sporadic, 94; no contact, 161) were obtained in Tunisia (2008-2009), and 99 of them presented other associated risk factors. Methicillin-resistant Staphylococcus aureus (MRSA) was detected in one of these 423 samples (0.24%), retrieved from a veterinarian. The MRSA isolate was mecA-positive, typed as ST80-t203-SCCmecIVc-agrIII, and contained tet(K), ant(6)-Ia, and aph(3′)-IIIa genes encoding tetracycline, streptomycin, and kanamycin resistance, respectively. This MRSA isolate also contained the lukF/lukS virulence gene encoding Panton-Valentine leukocidin. Fifty-four (12.8%) additional nasal samples contained methicillin-susceptible S. aureus (MSSA) and one isolate/sample was characterized. A high diversity of spa types (n=43; 4 new) and pulsed-field gel electrophoresis (PFGE) types (n=37) was detected among the 55 recovered S. aureus strains. The percentages of antimicrobial resistance/detected resistance genes were as follows: tetracycline [22%/tet(K)-tet(L)-tet(M)], erythromycin [5%/msrA], ciprofloxacin [14.5%], trimethoprim-sulfamethoxazole [2%/dfrA], streptomycin [11%/ant(6)-Ia], kanamycin [7%/aph(3′)-IIIa], amikacin [5%], and chloramphenicol [2%]. Four and two isolates carried the lukF/lukS and eta and/or etb genes, respectively, and always in individuals with contact with animals. Eleven isolates carried the tst gene and were recovered from individuals with different levels of contact with animals. © Springer-Verlag 2010.