Genetic environment of sul genes and characterization of integrons in Escherichia coli isolates from blood origin in a Spanish Hospital.

  1. Vinué, L. 1
  2. Sáenz, Y. 3
  3. Rojo-Bezares, B. 3
  4. Olarte, I. 2
  5. Undabeitia, E. 2
  6. Somalo, S. 1
  7. Zarazaga, M. 13
  8. Torres, C. 13
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Hospital San Pedro
    info

    Hospital San Pedro

    Logroño, España

    ROR https://ror.org/031va0421

  3. 3 Centro de Investigación Biomédica de La Rioja
    info

    Centro de Investigación Biomédica de La Rioja

    Logroño, España

    ROR https://ror.org/03vfjzd38

Show affiliations +
Journal:
International Journal of Antimicrobial Agents

ISSN: 0924-8579

Year of publication: 2010

Volume: 35

Issue: 5

Pages: 492-496

Type: Article

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DOI: 10.1016/J.IJANTIMICAG.2010.01.012 SCOPUS: 2-s2.0-77949652816 WoS: WOS:000275805700014 GOOGLE SCHOLAR

More publications in: International Journal of Antimicrobial Agents

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Evolución de la frecuencia y diversidad de integrones en escherichia coli de diferentes ecosistemas y su relación con la resistencia a los antimicrobianos

2006/00094/001

Abstract

The prevalence and characterisation of integrons and the genetic environment of sulphonamide resistance genes were studied in 135 Escherichia coli isolates recovered from blood cultures in a Spanish hospital during 2007. Class 1 and 2 integrons were identified in 54 isolates (intI1, 52 isolates; intI2, 1 isolate; and intI1+ intI2, 1 isolate). Of the 53 intI1-positive isolates, 36 (67.9%) contained the classic class 1 integron including the qacEΔ1- sul1 region, and 11 different gene cassette arrangements were demonstrated in 33 of these isolates. Seventeen intI1-positive isolates lacked the qacEΔ1- sul1 region, and 8 gene cassette arrangements were demonstrated in 12 of these isolates. Seventy-one isolates showed a sulphonamide-resistant phenotype, 63 of which contained sul genes. The sul1 gene was associated with intI1 in 36 of 42 sul1-positive isolates, and the sul3 gene was associated with non-classic class 1 integrons in 5 of 7 sul3-positive isolates. Finally, sul2 was found associated with strA- strB genes in 32 of 35 sul2-positive isolates, identifying 11 genetic structures, 1 of them presenting the IS. 150 element disrupting the strB gene; this structure was included in GenBank with accession no. FJ705354. Almost one-half of the E. coli isolates from blood cultures contained integrons and sul genes. Moreover, sul genes were detected in different structures, one of them new, and could be important determinants in antibiotic resistance dissemination. © 2010 Elsevier B.V. and the International Society of Chemotherapy.