MLST typing and genetic study of antibiotic resistance and virulence factors in vanA-containing Enterococcus recovered from buzzards (Buteo buteo).

  1. Radhouani, H. 2223
  2. Pinto, L. 2223
  3. Coelho, C. 2223
  4. Sargo, R. 2
  5. Araújo, C. 2223
  6. López, M. 1
  7. Torres, C. 1
  8. Igrejas, G. 22
  9. Poeta, P. 23
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

  3. 3 Center of Studies of Animal and Veterinary Sciences, Vila Real, Portugal
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Journal:
Letters in Applied Microbiology.

ISSN: 0266-8254

Year of publication: 2010

Volume: 50

Issue: 5

Pages: 537-541

Type: Article

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DOI: 10.1111/J.1472-765X.2010.02807.X PMID: 20141591 SCOPUS: 2-s2.0-77950465154 GOOGLE SCHOLAR

More publications in: Letters in Applied Microbiology.

Abstract

Aims: To analyse the occurrence of faecal carriage of vancomycin-resistant enterococci (VRE) in Buteo buteo and to study the associated resistance and virulence genes. Methods and Results: The presence of VRE was investigated in 33 faecal samples of B. buteo. Samples were seeded in Slanetz-Bartley agar plates supplemented with vancomycin for VRE recovery. Genes encoding antimicrobial resistance and virulence were studied by polymerase chain reaction. Vancomycin-resistant Enterococcus faecium isolates were characterized by multilocus sequence typing. VRE with an acquired mechanism of resistance (vanA genotype) were detected in 9% of samples analysed (Ent. faecium and Enterococcus durans). In addition, 27% of samples contained VRE with an intrinsic mechanism of resistance (Enterococcus gallinarum, vanC1). All vanA-containing isolates showed resistance to tetracycline and erythromycin and harboured the tet(M) and/or tet(L) genes, in addition to the ermB gene. The vat(E) and/or vat(D), cat(A) and aph(3′)-IIIa genes were identified in quinupristin- dalfopristin-, chloramphenicol-, and kanamycin-resistant vanA-containing strains, respectively. The sequence types ST273 and ST5 were identified in two vanA-positive Ent. faecium isolates, and the presence of hyl, gelE, cylA, cylL and cylM virulence genes and gelatinase activity were identified in Ent. faecium ST5 strain. Conclusions: The intestinal tract of B. buteo could be a reservoir of vanA-positive enterococci. Significance and Impact of the Study: First study focused to define the occurrence of vanA-containing Enterococcus strains in B. buteo. © 2010 The Authors. Journal compilation © 2010 The Society for Applied Microbiology.