Genomic and proteomic evaluation of antibiotic resistance in Salmonella strains.

  1. Pinto, L. 2
  2. Poeta, P. 2
  3. Vieira, S. 2
  4. Caleja, C. 2
  5. Radhouani, H. 2
  6. Carvalho, C. 2
  7. Vieira-Pinto, M. 2
  8. Themudo, P. 4
  9. Torres, C. 1
  10. Vitorino, R. 3
  11. Domingues, P. 3
  12. Igrejas, G. 2
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

  3. 3 Universidade de Aveiro
    info

    Universidade de Aveiro

    Aveiro, Portugal

    ROR https://ror.org/00nt41z93

  4. 4 National Laboratory of Veterinary Investigation, Lisbon, Portugal
Mostrar afiliaciones +
Revista:
Journal of Proteomics

ISSN: 1874-3919

Año de publicación: 2010

Volumen: 73

Número: 8

Páginas: 1535-1541

Tipo: Artículo

DOI: 10.1016/J.JPROT.2010.03.009 SCOPUS: 2-s2.0-77953120998 WoS: WOS:000279970800013 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Proteomics

Resumen

Using Salmonella strains identical to those present in the gastrointestinal tract of different animals we aim to determine and compare the proteome of two serotypes, Salmonella Typhimurium and Enteritidis recovered from faecal samples of wild boars and wild rabbits, respectively. The presence of genes responsible for antibiotic resistance was detected by PCR. Proteomes of the two distinct serotypes were determined using 2-DE in order to identify proteins associated with antibiotic resistance or virulence. Through 2-DE we obtained a total of 229 spots from both strains. All were suitable for MALDI-TOF/TOF and, in correlation with bioinformatic databases, allowed accurate identification and characterization of proteins. S. Enteritidis recovered from wild rabbits was sensitive to all the antibiotics tested in contrast to S. Typhimurium isolated from wild boars which presented a resistance phenotype to ampicillin, streptomycin and chloramphenicol. Nevertheless, despite the different ratio of proteins observed in each proteome according to their biological function, no significant difference was observed in the involvement of these proteins in pathogenicity. Bearing in mind that serotypes are related to infectious processes in humans and animals, it is important to explore the proteome of new strains which might serve as protein biomarkers for biological activity. © 2010 Elsevier B.V. All rights reserved.