Outbreak caused by a multi-resistant Klebsiella pneumoniae strain of new sequence type ST341 carrying new genetic environments of aac(6')-Ib-cr and qnrS1 genes in a neonatal intensive care unit in Spain.

  1. Ruiz, E. 1
  2. Rojo-Bezares, B. 2
  3. Sáenz, Y. 2
  4. Olarte, I. 4
  5. Esteban, I. 4
  6. Rocha-Gracia, R. 13
  7. Zarazaga, M. 1
  8. Torres, C. 12
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Centro de Investigación Biomédica de La Rioja
    info

    Centro de Investigación Biomédica de La Rioja

    Logroño, España

    ROR https://ror.org/03vfjzd38

  3. 3 Benemérita Universidad Autónoma de Puebla
    info

    Benemérita Universidad Autónoma de Puebla

    Heróica Puebla de Zaragoza, México

    ROR https://ror.org/03p2z7827

  4. 4 Hospital San Pedro
    info

    Hospital San Pedro

    Logroño, España

    ROR https://ror.org/031va0421

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Revista:
International Journal of Medical Microbiology

ISSN: 1438-4221

Any de publicació: 2010

Volum: 300

Número: 7

Pàgines: 464-469

Tipus: Article

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DOI: 10.1016/J.IJMM.2010.04.014 PMID: 20547103 SCOPUS: 2-s2.0-77957017259 WoS: WOS:000284338500008 GOOGLE SCHOLAR

Altres publicacions en: International Journal of Medical Microbiology

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Resum

An outbreak due to a Klebsiella pneumoniae clone occurred in a neonatal intensive care unit of a Spanish Hospital in which three newborns were infected (all with gestational age ≤29 weeks; two of them died) and seven were colonized (gestational age >32 weeks; none died). One K. pneumoniae strain per patient was further characterized. The 10 strains showed an indistinguishable pulsed-field-gel-electrophoresis pattern, were typed in the phylogenetic group KpI and were ascribed into a new sequence type registered as ST341. All 10 strains presented the same multiple-antibiotic-resistant phenotype, showed extended-spectrum-beta-lactamase production, and harbored the blaCTX-M-15, blaSHV-11, blaOXA-1, aac(6')-Ib-cr, qnrS1, aac(3)-II, aph(3')-Ia and aadA5 resistance genes. No class 1 or class 2 integrons were detected. The blaCTX-M-15 gene presented the following genetic environment: ISEcp1-blaCTX-M-15-orf477. These strains contained two copies of the aac(6')-Ib-cr gene included in the following new genetic environments: aac(3)-II-IS26-aac(6')-Ib-cr-blaOXA-1 and aac(3)-II-IS26-ΔcatB3-blaOXA-1-aac(6')-Ib-cr (registered at GenBank with accession numbers GQ438247 and GQ438248, respectively). The genetic environment of the qnrS1 gene (IS26-ΔISEcl2-qnrS1) (GenBank accession number GQ438249) was also not described previously. The aac(6')-Ib-cr, qnrS1, blaCTX-M-15, aac(3)-II, and blaOXA-1 genes, located in a plasmid of 33.5kb, could be transferred to Escherichia coli by transformation. © 2010 Elsevier GmbH.