High-affinity glucose transport in Aspergillus nidulans is mediated by the products of two related but differentially expressed genes

  1. Forment, Josep V. 23
  2. Flipphi, Michael. 24
  3. Ventura, Luisa. 2
  4. González, R. 25
  5. Ramón, D. 12
  6. MacCabe, A.P. 2
  1. 1 Universitat de València
    info

    Universitat de València

    Valencia, España

    ROR https://ror.org/043nxc105

  2. 2 Instituto de Agroquímica y Tecnología de los Alimentos
    info

    Instituto de Agroquímica y Tecnología de los Alimentos

    Valencia, España

    ROR https://ror.org/018m1s709

  3. 3 University of Cambridge
    info

    University of Cambridge

    Cambridge, Reino Unido

    ROR https://ror.org/013meh722

  4. 4 University of Paris-Sud
    info

    University of Paris-Sud

    Orsay, Francia

    ROR https://ror.org/028rypz17

  5. 5 Instituto de Ciencias de la Vid y del Vino
    info

    Instituto de Ciencias de la Vid y del Vino

    Logroño, España

    ROR https://ror.org/01rm2sw78

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Revue:
PLoS ONE

ISSN: 1932-6203

Année de publication: 2014

Volumen: 9

Número: 4

Type: Article

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DOI: 10.1371/JOURNAL.PONE.0094662 SCOPUS: 2-s2.0-84899685444 WoS: WOS:000335227400016 GOOGLE SCHOLAR lock_openAccès ouvert editor

D'autres publications dans: PLoS ONE

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Résumé

Independent systems of high and low affinity effect glucose uptake in the filamentous fungus Aspergillus nidulans. Low-affinity uptake is known to be mediated by the product of the mstE gene. In the current work two genes, mstA and mstC, have been identified that encode high-affinity glucose transporter proteins. These proteins' primary structures share over 90% similarity, indicating that the corresponding genes share a common origin. Whilst the function of the paralogous proteins is little changed, they differ notably in their patterns of expression. The mstC gene is expressed during the early phases of germination and is subject to CreA-mediated carbon catabolite repression whereas mstA is expressed as a culture tends toward carbon starvation. In addition, various pieces of genetic evidence strongly support allelism of mstC and the previously described locus sorA. Overall, our data define MstC/SorA as a high-affinity glucose transporter expressed in germinating conidia, and MstA as a high-affinity glucose transporter that operates in vegetative hyphae under conditions of carbon limitation. © 2014 Forment et al.