Relationships among gene expression and anthocyanin composition of malbec grapevine clones

  1. Muñoz, C. 2
  2. Gomez-Talquenca, S. 4
  3. Chialva, C. 2
  4. Ibáñez, J. 3
  5. Martinez-Zapater, J.M. 3
  6. Peña-Neira, A. 1
  7. Lijavetzky, D. 2
  1. 1 Universidad de Chile
    info

    Universidad de Chile

    Santiago de Chile, Chile

    ROR https://ror.org/047gc3g35

  2. 2 Universidad Nacional de Cuyo
    info

    Universidad Nacional de Cuyo

    Ciudad de Mendoza, Argentina

    ROR https://ror.org/05sn8wf81

  3. 3 Instituto de Ciencias de la Vid y del Vino
    info

    Instituto de Ciencias de la Vid y del Vino

    Logroño, España

    ROR https://ror.org/01rm2sw78

  4. 4 Instituto Nacional de Tecnología Agropecuaria
    info

    Instituto Nacional de Tecnología Agropecuaria

    Buenos Aires, Argentina

    ROR https://ror.org/04wm52x94

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Revista:
Journal of Agricultural and Food Chemistry

ISSN: 0021-8561

Año de publicación: 2014

Volumen: 62

Número: 28

Páginas: 6716-6725

Tipo: Artículo

DOI: 10.1021/JF501575M SCOPUS: 2-s2.0-84904430267 WoS: WOS:000339226700026 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Journal of Agricultural and Food Chemistry

Resumen

Anthocyanin profiles are commonly used for grapevine cultivar identification because it is currently accepted that this trait is closely related to their genetic characteristics. Nevertheless, the extent of the variation for the anthocyanin profiles among clones of the same cultivar has not yet been studied in depth. The relative concentration of anthocyanins of 131 Malbec clones grown in the same vineyard was investigated by HPLC-DAD and the use of comprehensive statistic procedures. Complementarily, the expression level of structural and regulatory genes was studied via real time polymerase chain reaction. Significant variation was identified among the profiles of the clones, mainly due to variations in the amounts of malvidin derivatives. Finally, the differential expression in F3′5′H, OMT1 and AM2 genes seems to be related to the malvidin content variation. This work shows the existence of variation for the anthocyanin profiles among clones from the same grapevine cultivar and the putative involvement of genes related to hydroxylation, methylation, and transport of anthocyanins on the basis of such variation. © 2014 American Chemical Society.