Detection of antibiotic resistant enterococci and Escherichia coli in free range Iberian Lynx (Lynx pardinus)

  1. Gonçalves, A. 3
  2. Igrejas, G. 3
  3. Radhouani, H. 3
  4. Santos, T. 3
  5. Monteiro, R. 3
  6. Pacheco, R. 3
  7. Alcaide, E. 2
  8. Zorrilla, I. 2
  9. Serra, R. 4
  10. Torres, C. 1
  11. Poeta, P. 3
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Center for Analysis and Diagnosis of Wildlife (CAD), Parque Nacional de Doñana, Spain
  3. 3 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

  4. 4 National Centre for Captive Breeding of the Iberian Lynx, Silves, Portugal
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Revista:
Science of The Total Environment

ISSN: 0048-9697

Año de publicación: 2013

Volumen: 456-457

Páginas: 115-119

Tipo: Artículo

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DOI: 10.1016/J.SCITOTENV.2013.03.073 SCOPUS: 2-s2.0-84876379120 WoS: WOS:000320343700014 GOOGLE SCHOLAR

Otras publicaciones en: Science of The Total Environment

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2013/00001/001

Resumen

Thirty fecal samples from wild specimens of Iberian lynx were collected and analyzed for Enterococcus spp. (27 isolates) and Escherichia coli (18 isolates) recovery. The 45 isolates obtained were tested for antimicrobial resistance, molecular mechanisms of resistance, and presence of virulence genes. Among the enterococci, Enterococcus faecium and Enterococcus hirae were the most prevalent species (11 isolates each), followed by Enterococcus faecalis (5 isolates). High percentages of resistance to tetracycline and erythromycin (33% and 30%, respectively) were detected among enterococcal isolates. The tet(M) and/or tet(L), erm(B), aac(6')-Ie-aph(2″)-Ia, ant(6)-Ia, or aph(3')-IIIa genes were detected among resistant enterococci. Virulence genes were detected in one E. faecalis isolate (cpd, cylB, and cylL) and one E. hirae isolate (cylL). High percentages of resistance were detected in E. coli isolates to tetracycline (33%), streptomycin (28%), nalidixic acid (28%), and sulfamethoxazole-trimethoprim (SXT, 22%). Additionally, the blaTEM, tet(A), aadA, cmlA, and different combinations of sul genes were detected among most ampicillin, tetracycline, streptomycin, chloramphenicol and SXT-resistant isolates, respectively. Two isolates contained a class 1 integron with the gene cassette arrays dfrA1+aadA1 and dfrA12+aadA2. The E. coli isolates were ascribed to phylo-groups A (n=5); B1 (n=4); B2 (n=6), and D (n=3), with the virulence gene fimA present in all E. coli isolates. This study found resistance genes in wild specimens of Iberian lynx. Thus, it is important to notice that multiresistant bacteria have reached species as rare and completely non-synanthropic as the Iberian lynx. Furthermore, the susceptibility of this endangered species to bacterial infection may be affected by the presence of these virulence and resistance genes.© 2013 Elsevier B.V.