Molecular characterization of multiresistant Escherichia coli producing or not extended-spectrum beta-lactamases

  1. Ruiz Del Castillo, B. 5
  2. Vinué, L. 1
  3. Román, E.J. 5
  4. Guerra, B. 3
  5. Carattoli, A. 4
  6. Torres, C. 1
  7. Martínez-Martínez, L. 25
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Universidad de Cantabria
    info

    Universidad de Cantabria

    Santander, España

    ROR https://ror.org/046ffzj20

  3. 3 Federal Institute for Risk Assessment
    info

    Federal Institute for Risk Assessment

    Berlín, Alemania

    ROR https://ror.org/03k3ky186

  4. 4 Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita, Rome, Italy
  5. 5 Hospital Universitario Marqués de Valdecilla
    info

    Hospital Universitario Marqués de Valdecilla

    Santander, España

    ROR https://ror.org/01w4yqf75

Mostrar afiliaciones +
Revista:
BMC Microbiology

ISSN: 1471-2180

Año de publicación: 2013

Volumen: 13

Número: 1

Páginas: 1-12

Tipo: Artículo

DOI: 10.1186/1471-2180-13-84 PMID: 23586437 SCOPUS: 2-s2.0-84876082029 WoS: WOS:000318623100001 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: BMC Microbiology

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

Background: The prevalence and type of plasmids, resistance genes and integrons carried by two collections of multiresistant E. coli producing or not extended-spectrum beta-lactamases have been compared. Rep-PCR was used to determine the clonal relationship of the organisms. Plasmids were classified according to their incompatibility. Class 1 and Class 2 integrons and antibiotic resistance genes were analysed by PCR and sequencing. Results: Both collections of organisms contained a large diversity of unrelated strains with some clones distributed in both groups of isolates. Large plasmids were identified in the two groups of organisms. Plasmids with replicons repK and repColE were more frequent among ESBL-producing isolates, while repFIA, repFII and repA/C replicons were more frequent in isolates lacking ESBL. Conjugative plasmids with repK and repA/C replicons coded for CTX-M-14 and CMY-2 beta-lactamases, respectively. No significant differences were observed in the distribution of class 1 and class 2 integrons among multiresistant E. coli producing or not ESBL, and dfrA17-ant(3[prime][prime])-Ie was the cassette arrangement most commonly found. Conclusions: In the concrete temporal and geographical context of this study, multiresistant E. coli producing ESBL or other mechanisms of resistance were largely clonally diverse and present some differences in the types of harboured plasmids. Still, some clones were found in both ESBL-producing and --lacking isolates.