Species distribution, antibiotic resistance and virulence traits in canine and feline enterococci in Tunisia

  1. Ben Said, L. 3
  2. Dziri, R. 3
  3. Sassi, N. 3
  4. Lozano, C. 1
  5. Ben Slama, K. 3
  6. Ouzari, I. 3
  7. Torres, C. 12
  8. Klibi, N. 3
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Centro de Investigación Biomédica de La Rioja
    info

    Centro de Investigación Biomédica de La Rioja

    Logroño, España

    ROR https://ror.org/03vfjzd38

  3. 3 Université de Tunis El Manar
    info

    Université de Tunis El Manar

    Túnez, Túnez

    ROR https://ror.org/029cgt552

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Revista:
Acta Veterinaria Hungarica

ISSN: 0236-6290

Año de publicación: 2017

Volumen: 65

Número: 2

Páginas: 173-184

Tipo: Artículo

DOI: 10.1556/004.2017.018 SCOPUS: 2-s2.0-85020765762 WoS: WOS:000403315000003 GOOGLE SCHOLAR

Otras publicaciones en: Acta Veterinaria Hungarica

Resumen

In order to investigate the possible role of dogs and cats in the carriage and potential dissemination of resistant enterococci, seventy faecal samples from dogs and cats were tested for enterococci. Fifty-eight enterococci were recovered. Isolates were identified as Enterococcus faecium (n = 31) and E. faecalis (n = 14) E. durans (n = 6), E. casseliflavus (n = 2), E. hirae and E. gallinarum (2 isolates each). Enterococcal isolates showed resistance to ciprofloxacin (n = 35), erythromycin (n = 31), tetracycline (n = 25), kanamycin (n = 15), streptomycin (n = 13), pristi-namycin (n = 11), gentamicin (n = 10), chloramphenicol (n = 8), and linezolid (n = 6). The gene erm(B) was detected in 22 out of 31 erythromycin-resistant enterococci. All tetracycline-resistant enterococci carried tet(M) and/or tet(L) genes. The gene aac(6′)-Ie-aph(2")-Ia was identified in five of high-level gentamicin-resistant isolates, the genes aph(3′)-IIIa and/or aac(6′)-le-aph(2")-Ia in eleven high-level kanamycin-resistant isolates and the gene ant(6)-Ia in eleven high-level streptomycin-resistant isolates. Only one strain harboured cat(A) gene, and five strains contained vat(E) or vat(D) genes. Virulence genes gel(E) (21 strains), esp (11 strains) and cylA/cylB (5 strains) were detected. High genetic diversity was demonstrated among E. faecium isolates by pulsed-field gel electrophoresis (PFGE). Dogs and cats can be carriers of antibiotic-resistant enterococci in their faeces that could shed into the household environment. © 2017 The Authors.