Occurrence and characterization of stx and/or eae-positive Escherichia coli isolated from wildlife, including a typical EPEC strain from a wild boar

  1. Alonso, C.A. 1
  2. Mora, A. 2
  3. Díaz, D. 2
  4. Blanco, M. 2
  5. González-Barrio, D. 3
  6. Ruiz-Fons, F. 3
  7. Simón, C. 4
  8. Blanco, J. 2
  9. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Universidade de Santiago de Compostela
    info

    Universidade de Santiago de Compostela

    Santiago de Compostela, España

    ROR https://ror.org/030eybx10

  3. 3 Instituto de Investigación en Recursos Cinegéticos
    info

    Instituto de Investigación en Recursos Cinegéticos

    Ciudad Real, España

    ROR https://ror.org/0140hpe71

  4. 4 Universidad de Zaragoza
    info

    Universidad de Zaragoza

    Zaragoza, España

    ROR https://ror.org/012a91z28

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Journal:
Veterinary Microbiology

ISSN: 0378-1135

Year of publication: 2017

Volume: 207

Pages: 69-73

Type: Article

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DOI: 10.1016/J.VETMIC.2017.05.028 SCOPUS: 2-s2.0-85020681118 WoS: WOS:000407981400012 GOOGLE SCHOLAR

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Abstract

Shiga toxin-producing E. coli (STEC) and enteropathogenic E. coli (EPEC) strains are food-borne pathogens associated with acute diarrhea. Haemolytic-uremic syndrome (HUS) is often a complication of STEC infection. In order to examine the occurrence, serotypes, virulence and antimicrobial-resistance profiles of STEC and EPEC in wildlife, 326 faecal E. coli strains from 304 clinically healthy animals were analyzed. For this approach stx1, stx2 and eae genes, as well as accessory virulence determinants (ehx, hlyA, saa, tia, bfp, subAB) were PCR-screened and sequenced. Serotyping was performed employing all available O (O1–O185) and H (H1-H56) antisera. Genetic diversity was analyzed by XbaI-PFGE and phylotyping. Thirteen STEC (4.3%) and 10 EPEC (3.3%) were identified among 12 deer, 3 mouflon, 6 wild boars and 2 birds. Nine STEC showed seropathotypes B (O145:[H28]) and C (O22:H8, O128:[H2]) associated with HUS, and D (O110:H28, O146:H21, O146:[H28], ONT:H8) associated with human diarrhea. Although most isolates harbored stx2b and stx1c variants, stx2a and stx1a (related with severe disease) were also detected. Additionally, the eae gene was present in one stx2a–positive O145:[H28] STEC from a deer and 11 STEC harbored subAB genes (mainly the subAB2 variant). EPEC isolates showed 7 different intimin variants (β1, β2, γ1, ε1, ζ1, ι1-A, κ). Interestingly, the O49:[H10] eae-κ EPEC isolated from a wild boar was bfpA-positive showing a combination of serotype/virulence profile previously detected among human clinical tEPEC. Based on present results, wild ruminants, wild boars and to a lesser extent birds would be carriers of potentially pathogenic STEC and EPEC strains. © 2017 Elsevier B.V.