Genetic characterization of Staphylococcus aureus isolated from nasal samples of healthy ewes in Tunisia. High prevalence of CC130 and CC522 lineages

  1. Ben Said, M. 23
  2. Abbassi, M.S. 3
  3. Gómez, P. 1
  4. Ruiz-Ripa, L. 1
  5. Sghaier, S. 23
  6. El Fekih, O. 3
  7. Hassen, A. 2
  8. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Centre de Recherches et des Technologies des Eaux
    info

    Centre de Recherches et des Technologies des Eaux

    Nabeul, Túnez

    ROR https://ror.org/05v1dya15

  3. 3 Université de Tunis El Manar
    info

    Université de Tunis El Manar

    Túnez, Túnez

    ROR https://ror.org/029cgt552

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Revista:
Comparative Immunology, Microbiology and Infectious Diseases

ISSN: 0147-9571

Año de publicación: 2017

Volumen: 51

Páginas: 37-40

Tipo: Artículo

DOI: 10.1016/J.CIMID.2017.03.002 SCOPUS: 2-s2.0-85017386678 WoS: WOS:000405274300006 GOOGLE SCHOLAR

Otras publicaciones en: Comparative Immunology, Microbiology and Infectious Diseases

Repositorio institucional: lock_openAcceso abierto Postprint

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Resumen

Staphylococcus aureus is a versatile bacterium, which can infect or colonize a variety of host species. The objective of this study was to characterize S. aureus isolates recovered from nasal swabs of 167 healthy ewes sampled from 12 farms in different areas of Tunisia during the period of 2014–2015. Genetic lineages, virulence factors and antibiotic resistance mechanisms were determined for recovered isolates. S. aureus was detected in 45 out of 167 tested samples (26.9%). All isolates were methicillin-susceptible (MSSA) and the majority of them were susceptible to tested antibiotics with few exceptions (% of resistance): penicillin (8.8), ciprofloxacin (4.4), and tobramycin or tetracycline (2.2, each). Twelve different spa types were detected (t15098, t15099, t1773, t3576, t1534, t5428, t3750, t5970 t254, t2883, t127 and t933), two of them were new (t15098 and t15099). S. aureus isolates were ascribed to agrI (n = 23), agrII (n = 1) and agrIII (n = 20), and one was non-typeable. According to the sequence-type (ST) determined and/or the spa-type detected, the 45 S. aureus isolates were assigned to six clonal complexes, with CC522 (44.4%) and CC130 (37.7%) being the most common lineages. Twenty-one (46.6%) and two (4.2%) isolates harbored the tst and eta genes encoding TSST-1 and ETA, respectively. In conclusion, nares of healthy ewes could be a reservoir of MSSA CC522 and CC130, lineages associated with TSST-1 and ETA that might represent a risk to human health. © 2017 Elsevier Ltd