Clonal diversity of extended-spectrum beta-lactamase producing Escherichia coli isolates in fecal samples of wild animals

  1. Cristóvão, F. 23
  2. Alonso, C.A. 2
  3. Igrejas, G. 35
  4. Sousa, M. 3
  5. Silva, V. 3
  6. Pereira, J.E. 3
  7. Lozano, C. 12
  8. Cortés-Cortés, G. 24
  9. Torres, C. 12
  10. Poeta, P. 35
  1. 1 Centro de Investigación Biomédica de La Rioja
    info

    Centro de Investigación Biomédica de La Rioja

    Logroño, España

    ROR https://ror.org/03vfjzd38

  2. 2 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  3. 3 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

  4. 4 Benemérita Universidad Autónoma de Puebla
    info

    Benemérita Universidad Autónoma de Puebla

    Heróica Puebla de Zaragoza, México

    ROR https://ror.org/03p2z7827

  5. 5 Universidade Nova de Lisboa
    info

    Universidade Nova de Lisboa

    Lisboa, Portugal

    ROR https://ror.org/02xankh89

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Revista:
FEMS Microbiology Letters

ISSN: 0378-1097

Ano de publicación: 2017

Volume: 364

Número: 5

Tipo: Artigo

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DOI: 10.1093/FEMSLE/FNX039 SCOPUS: 2-s2.0-85018256060 GOOGLE SCHOLAR

Outras publicacións en: FEMS Microbiology Letters

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Resumo

The clonal diversity of extended-spectrum-β-lactamase (ESBL)-producing Escherichia coli isolates from nine different species of wild animals from distinct regions of Portugal and Spain and their content in replicon plasmids were analyzed. Among the initial 53 ESBL-producing E. coli isolates that were studied (from previous studies), 28 were selected, corresponding to different animal origins with distinct ESBL types and pulsed-field gel electrophoresis (PFGE) patterns. These 28 isolates produced different ESBLs ascribed to the following families: CTX-M, SHV and TEM. The isolates were classified into three phylogenetic groups: B1 (n = 11), A (n = 10) and D (n = 7). The seven E. coli of phylogroup D were then typed by multilocus sequence typing and ascribed to four distinct sequence types: ST117, ST115, ST2001 and ST69. The clonal diversity and relationship between isolates was studied by PFGE. Lastly, the plasmids were analyzed according to their incompatibility group using the PCR-based-replicon-typing scheme. A great diversity of replicon types was identified, with up to five per isolate. Most of the CTX-M-1 and SHV-12 producing E. coli isolates carried IncI1 or IncN replicons. The diversity of ESBL-producing E. coli isolates in wild animals, which can be disseminated in the environment, emphasizes the environmental and health problems that we face nowadays. © FEMS 2017. All rights reserved.