Characterization of methicillin- and linezolid-resistant Staphylococcus epidermidis and S. haemolyticus strains in a Spanish hospital [Caracterización de cepas de Staphylococcus epidermidis y S. haemolyticus resistentes a meticilina y linezolid en un hospital español]

  1. Lozano, C. 1
  2. Aspiroz, C. 2
  3. Gómez-Sanz, E. 1
  4. Tirado, G. 3
  5. Fortuño, B. 2
  6. Zarazaga, M. 1
  7. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Unidad de Microbiología, Hospital Royo Villanova, Zaragoza, Spain
  3. 3 Servicio de Medicina Intensiva, Hospital Royo Villanova, Zaragoza, Spain
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Revista:
Enfermedades Infecciosas y Microbiologia Clinica

ISSN: 0213-005X

Año de publicación: 2013

Volumen: 31

Número: 3

Páginas: 136-141

Tipo: Artículo

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DOI: 10.1016/J.EIMC.2012.08.006 SCOPUS: 2-s2.0-84875170288 WoS: WOS:000316579700003 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Enfermedades Infecciosas y Microbiologia Clinica

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Proyectos relacionados

Detección y caracterización genética de Staphylococcus aureus resistente a meticilina en distintos ecosistemas: humanos, animales, alimentos y medioambiente. Impicaciones en salud humana.

2009/00204/001

Resumen

Introduction: Linezolid resistance is mainly due to mutations in the 23S rRNA target. The aim of this study was to characterize linezolid and methicillin resistant Staphylococcus epidermidis (SE-LMR) and S. haemolyticus (SH-LMR) strains detected in a Spanish hospital. Methods: SE-LM R and SH-LMR strains obtained in the period June 2009-August 2011 in a second level hospital were recorded along with the epidemiological characteristics of the patients. These strains were typed, and their resistance, phenotype, genotype and the factors determining their virulence were analysed. Results: Linezolid resistance was explained by the presence of G2603T mutation (23S rRNA) and aminoacid changes in L3 and L4 ribosomal proteins. The 25 SE-LMR strains belonged to sequence type ST2, presented SCCmec type III, and two different PFGE patterns. The two SH-LMR strains showed non-typeable SCCmec. SE-LMR strains harboured the resistance genes aac(6')-aph(2"), and dfrS1. SH-LM R strains contained these genes and the gene erm(C). No lincomycin resistance mechanism was identified in SE-LMR strains regardless of showing lincomycin resistance and diminished susceptibility to clindamycin. Conclusions: Linezolid resistance is of concern in hospitals, and requires continued vigilance. Several linezolid resistance mechanisms (mutation in 23S RNAr and amino acid changes in L3 and L4) were identified in this study. © 2012 Elsevier España, S.L. All rights reserved.