First Detection of CTX-M-1, CMY-2, and QnrB19 Resistance Mechanisms in Fecal Escherichia coli Isolates from Healthy Pets in Tunisia

  1. Sallem, R.B. 3
  2. Gharsa, H. 3
  3. Slama, K.B. 3
  4. Rojo-Bezares, B. 2
  5. Estepa, V. 1
  6. Porres-Osante, N. 12
  7. Jouini, A. 3
  8. Klibi, N. 3
  9. Sáenz, Y. 2
  10. Boudabous, A. 3
  11. Torres, C. 12
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Centro de Investigación Biomédica de La Rioja
    info

    Centro de Investigación Biomédica de La Rioja

    Logroño, España

    ROR https://ror.org/03vfjzd38

  3. 3 Université de Tunis El Manar
    info

    Université de Tunis El Manar

    Túnez, Túnez

    ROR https://ror.org/029cgt552

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Revista:
Vector-Borne and Zoonotic Diseases

ISSN: 1530-3667

Año de publicación: 2013

Volumen: 13

Número: 2

Páginas: 98-102

Tipo: Artículo

DOI: 10.1089/VBZ.2012.1047 SCOPUS: 2-s2.0-84873363787 WoS: WOS:000314364100004 GOOGLE SCHOLAR

Otras publicaciones en: Vector-Borne and Zoonotic Diseases

Resumen

Our objective was to analyze the carriage rate of extended-spectrum β-lactamase (ESBL)-and plasmidic AmpC β-lactamase (pAmpC)-producing Escherichia coli isolates in fecal samples of healthy pets (dogs and cats) and to characterize the recovered isolates for the presence of other resistance genes and integrons. Eighty fecal samples of healthy pets were inoculated in MacConkey agar plates supplemented with cefotaxime (2 μg/mL) for cefotaxime-resistant (CTXR) E. coli recovery. CTXR E. coli isolates were detected in 14 of the 80 fecal samples (17.5%) and the following β-lactamase genes (number of isolates) were detected: blaCTX-M-1 (8), blaCTX-M-1+blaTEM-1b (3), bla CTX-M-1+blaTEM-1c (1), blaCTX-M-1+bla TEM-135 (1), and blaCMY-2+blaTEM-1b (1). The 14 E. coli were distributed into the phylogroups B1 (6 isolates), A (5), and D (3). The qnrB19 gene was detected in one CTX-M-1-producing strain of phylogroup D. Five isolates contained class 1 integrons with the following arrangements: dfrA17-aadA5 (2 isolates), dfrA1-aadA1 (1), and dfrA17-aadA5/dfrA1-aadA1 (2 isolates). The virulence genes fimA and/or aer were detected in all CTX R strains. In this study, the pet population harbored β-lactamase and quinolone resistance genes of special interest in human health that potentially could be transmitted to humans in close contact with them. © Copyright 2013, Mary Ann Liebert, Inc. 2013.