Detection of aminoglycoside-penicillin synergy against Enterococcus faecium with high-content aminoglycoside disks.

  1. Torres, C. 1
  2. Tenorio, C. 1
  3. Lantero, M. 2
  4. Zarazaga, M. 1
  5. Baquero, F. 3
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Hospital San Pedro
    info

    Hospital San Pedro

    Logroño, España

    ROR https://ror.org/031va0421

  3. 3 Hospital Ramón y Cajal
    info

    Hospital Ramón y Cajal

    Madrid, España

    ROR https://ror.org/050eq1942

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Journal:
European Journal of Clinical Microbiology and Infectious Diseases

ISSN: 0934-9723

Year of publication: 1995

Volume: 14

Issue: 10

Pages: 878-882

Type: Article

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DOI: 10.1007/BF01691494 PMID: 8605901 SCOPUS: 2-s2.0-0028786197 WoS: WOS:A1995TF55000006 GOOGLE SCHOLAR

More publications in: European Journal of Clinical Microbiology and Infectious Diseases

Abstract

Thirty-seven Enterococcus faecium strains were screened for high-level aminoglycoside resistance with an agar diffusion test using high-content aminoglycoside disks (300 μg of streptomycin and 120 μg of gentamicin, tobramycin, kanamycin or amikacin). The inhibition zones obtained were correlated with results of time-kill penicillin-aminoglycoside synergy studies. An 11 mm breakpoint differentiated strains susceptible or resistant to the synergy of streptomycin plus penicillin. Irrespective of the inhibition zones obtained with tobramycin and kanamycin disks, Enterococcus faecium strains never showed synergy with penicillin in combination with these aminoglycosides. Penicillin-amikacin synergy cannot be predicted by the amikacin disks. Nevertheless, even though kanamycin disks do not predict penicillin-kanamycin synergy, they can be used to predict penicillin-kanamycin synergy. In summary, high-content streptomycin, gentamicin and kanamycin disks can be used to predict the susceptibility of Enterococcus faecium strains to the synergistic combination of penicillin plus one of the aminoglycosides (streptomycin, gentamicin or amikacin, respectively).