Multilocus sequence Typing Scheme for Enterococucus faecalis Reveals Hospital-Adapted genetic Complexes in Background of High Rates of Recombination

  1. Ruiz-Garbajosa, P. 5
  2. Bonten, M.J.M. 4
  3. Robinson, D.A. 3
  4. Top, J. 4
  5. Nallapareddy, S.R. 1
  6. Torres, C. 2
  7. Coque, T.M. 5
  8. Cantón, R. 5
  9. Baquero, F. 5
  10. Murray, B.E. 1
  11. Del Campo, R. 5
  12. Willems, R.J.L. 4
  1. 1 University of Texas System
    info

    University of Texas System

    Austin, Estados Unidos

    ROR https://ror.org/01gek1696

  2. 2 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  3. 3 New York Medical College
    info

    New York Medical College

    Valhalla, Estados Unidos

    ROR https://ror.org/03dkvy735

  4. 4 University Medical Center Utrecht
    info

    University Medical Center Utrecht

    Utrecht, Holanda

    ROR https://ror.org/0575yy874

  5. 5 Hospital Ramón y Cajal
    info

    Hospital Ramón y Cajal

    Madrid, España

    ROR https://ror.org/050eq1942

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Revista:
Journal of Clinical Microbiology

ISSN: 0095-1137

Año de publicación: 2006

Volumen: 44

Número: 6

Páginas: 2220-2228

Tipo: Artículo

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DOI: 10.1128/JCM.02596-05 PMID: 16757624 SCOPUS: 2-s2.0-33745136790 WoS: WOS:000238332900044 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Clinical Microbiology

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Resumen

A multilocus sequence typing (MLST) scheme based on seven housekeeping genes was used to investigate the epidemiology and population structure of Enterococcus faecalis. MLST of 110 isolates from different sources and geographic locations revealed 55 different sequence types that grouped into four major clonal complexes (CC2, CC9, CC10, and CC21) by use of eBURST. Two of these clonal complexes, CC2 and CC9, are particularly fit in the hospital environment, as CC2 includes the previously described BVE clonal complex identified by an alternative MLST scheme and CC9 includes exclusively isolates from hospitalized patients. Identical alleles were found in genetically diverse isolates with no linkage disequilibrium, while the different MLST loci gave incongruent phylogenetic trees. This demonstrates that recombination is an important mechanism driving genetic variation in E. faecalis and suggests an epidemic population structure for E. faecalis. Our novel MLST scheme provides an excellent tool for investigating local and short-term epidemiology as well as global epidemiology, population structure, and genetic evolution of E. faecalis. Copyright © 2006, American Society for Microbiology. All Rights Reserved.