Supercritical fluid extraction of phenol compounds from olive leaves
- Le Floch, F. 1
- Tena, M.T. 1
- Ríos, A. 1
- Valcárcel, M. 1
-
1
Universidad de Córdoba
info
ISSN: 0039-9140
Año de publicación: 1998
Volumen: 46
Número: 5
Páginas: 1123-1130
Tipo: Artículo
Otras publicaciones en: Talanta (Oxford)
Resumen
A clean, highly selective supercritical fluid extraction (SFE) method for the isolation of phenols from olive leaf samples was examined. Total phenol extracts were determined using the Folin-Ciocalteu reagent. Dried, ground, sieved olive leaf samples (30 mg) are subjected to SFE, using carbon dioxide modified with 10% methanol at 334 bar, 100°C (CO2 density 0.70 g ml-1) at a liquid flow-rate of 2 ml min-1 for 140 min. Diatomaceous earth is used to reduce the void volume of the extraction vessel. The influence of extraction variables such as modifier content, pressure, temperature, flow-rate, extraction time, and collection/elution variables, were studied. Supercritical fluid extracts were screened for acid compounds such as carboxylic acids and phenols using Electrospray-MS (in the negative ionization mode). SFE was found to produce higher phenol recoveries than sonication in liquid solvents such as n-hexane, diethyl ether and ethyl acetate. However, the extraction yield obtained was only 45%, using liquid methanol.