β-Lactamases, transferable quinolone resistance determinants, and class 1 integron-mediated antimicrobial resistance in human clinical Salmonella enterica isolates of non-Typhimurium serotypes

  1. Pérez-Moreno, M.O. 45
  2. Picó-Plana, E. 45
  3. de Toro, M. 12
  4. Grande-Armas, J. 45
  5. Quiles-Fortuny, V. 45
  6. Pons, M.J. 3
  7. Gomes, C. 3
  8. Sáenz, Y. 2
  9. Torres, C. 12
  10. Ruiz, J. 3
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Centro de Investigación Biomédica de La Rioja
    info

    Centro de Investigación Biomédica de La Rioja

    Logroño, España

    ROR https://ror.org/03vfjzd38

  3. 3 Universitat de Barcelona
    info

    Universitat de Barcelona

    Barcelona, España

    ROR https://ror.org/021018s57

  4. 4 Institut d'Investigació Sanitària Pere Virgili
    info

    Institut d'Investigació Sanitària Pere Virgili

    Reus, España

  5. 5 Servicio de Análisis Clínicos, Hospital de Tortosa Verge de la Cinta, Tortosa, Spain
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Revista:
International Journal of Medical Microbiology

ISSN: 1438-4221

Año de publicación: 2013

Volumen: 303

Número: 1

Páginas: 25-31

Tipo: Artículo

DOI: 10.1016/J.IJMM.2012.11.003 SCOPUS: 2-s2.0-84872929707 WoS: WOS:000317874300004 GOOGLE SCHOLAR

Otras publicaciones en: International Journal of Medical Microbiology

Resumen

This work investigates the occurrence and features of class 1 integrons and the presence of transferable quinolone resistance determinants (TQRD) among 382 clinical Salmonella enterica isolates of non-Typhimurium serotypes as well as the β-lactamases produced by amoxicillin-resistant isolates. These isolates were recovered in 2001 and from 2004 to 2009 from patients from the health region of Terres de l'Ebre (Catalonia, Spain) and comprised 41 different serotypes, mostly of serovar Enteritidis (. n=. 272), being 16.5% multidrug-resistant (MDR). Among the 93 amoxicillin-resistant isolates, 84 produced TEM-1,4 produced an extended-spectrum β-lactamase (CTX-M-9 in one S. Grumpensis and in one S. Virchow, CTX-M-15 in S. Kapemba, and SHV-12 in S. Enteritidis), one produced DHA-1 (. S. Newport), and 4 did not present any of the investigated β-lactamases. TQRD were found in 2 isolates (. qnrA1 in CTX-M-9-producing S. Grumpensis and qnrB4 in DHA-1-producing S. Newport). Overall, 35 isolates (9.2% of all isolates and 54% of MDR isolates) belonging to 15 different serotypes carried class 1 integrons that were transferred by conjugation in 17 isolates. Eleven distinct cassette arrangements were identified, with dfrA1-aadA1, dfrA17-aadA5, and dfrA12-orfF-aadA2 being the most prevalent and widely distributed ones. Atypical sul3-associated integrons were detected in 5 isolates of serotypes Rissen and Enteritidis. Moreover, the presence of integrons in the serotypes Kapemba, Mikawasima, and [9,12:Iv:i:-], of the estX-psp (linked to sul3) and aadA13-sat cassette arrangements in S. enterica, of extended-spectrum β-lactamases in S. Kapemba and S. Grumpensis, and of TQRD in S. Grumpensis is reported here for the first time. © 2012 Elsevier GmbH.