Species distribution, antibiotic resistance and virulence traits in enterococci from meat in Tunisia

  1. Klibi, N. 2
  2. Said, L.B. 2
  3. Jouini, A. 2
  4. Slama, K.B. 2
  5. López, M. 1
  6. Sallem, R.B. 2
  7. Boudabous, A. 2
  8. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Université de Tunis El Manar
    info

    Université de Tunis El Manar

    Túnez, Túnez

    ROR https://ror.org/029cgt552

Journal:
Meat Science

ISSN: 0309-1740

Year of publication: 2013

Volume: 93

Issue: 3

Pages: 675-680

Type: Article

beta Ver similares en nube de resultados
DOI: 10.1016/J.MEATSCI.2012.11.020 SCOPUS: 2-s2.0-84870885625 WoS: WOS:000314735100044 GOOGLE SCHOLAR

More publications in: Meat Science

Abstract

Antimicrobial resistance and the mechanisms implicated were studied in 119 enterococci from 105 meat samples from Tunisian markets. Almost 24.5% of recovered enterococci showed resistance against four or more antimicrobial agents and these isolates were identified to the species level. Enterococcus faecalis was the most prevalent species (41%). High percentages of erythromycin and tetracycline resistances were found among our isolates, and lower percentages were identified to aminoglycosides, ciprofloxacin and chloramphenicol. All tetracycline-resistant isolates carried the tet(M) and/or tet(L) genes. The erm(B) gene was detected in 78.5% of erythromycin-resistant isolates, ant(6)-Ia gene in 58.8% of streptomycin-resistant isolates, and cat(A) gene in one chloramphenicol-resistant isolate. Forty-eight isolates carried the gelE gene and exhibited gelatinase activity. The hyl and esp genes were detected in one and three Enterococcus faecium isolates, respectively. Streptomycin-resistant isolates showed a high genetic diversity by PFGE and MLST. Meat might play a role in the spread through the food chain of enterococci with these virulence and resistance characteristics to humans. © 2012 Elsevier Ltd.