Community fecal carriage of broad-spectrum cephalosporin-resistant Escherichia coli in Tunisian children

  1. Ferjani, S. 3
  2. Saidani, M. 23
  3. Hamzaoui, Z. 3
  4. Alonso, C.A. 1
  5. Torres, C. 1
  6. Maamar, E. 3
  7. Slim, A.F. 2
  8. Boutiba, B.B.I. 23
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Hôpital Charles-Nicolle
    info

    Hôpital Charles-Nicolle

    Túnez, Túnez

    ROR https://ror.org/02f6ghw27

  3. 3 Université de Tunis El Manar
    info

    Université de Tunis El Manar

    Túnez, Túnez

    ROR https://ror.org/029cgt552

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Journal:
Diagnostic Microbiology and Infectious Disease

ISSN: 0732-8893

Year of publication: 2017

Volume: 87

Issue: 2

Pages: 188-192

Type: Article

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DOI: 10.1016/J.DIAGMICROBIO.2016.03.008 SCOPUS: 2-s2.0-85006797797 WoS: WOS:000393353000021 GOOGLE SCHOLAR

More publications in: Diagnostic Microbiology and Infectious Disease

Abstract

The spread of extended spectrum β-lactamases (ESBL) and plasmid mediated AmpC β-lactamases (pAmpC) was evaluated in Escherichia coli strains collected from the intestinal microbiota of healthy children in Tunisia. The carriage rate of CTXRE. coli was 6.6% (7 of 105 samples) and one strain/sample was further characterized (7 isolates). These isolates harbored blaCTX-M-1 (n = 4), blaCTX-M-15 (n = 2), and blaCMY-2 gene (n = 1), which were usually located on FIB replicon type and carried class 1 integrons. The acc(6′)-Ib-cr variant was identified in one isolate that harbored blaCTX-M-15. CTXRE. coli isolates were genetically unrelated and belonged to B1 (n = 3/ST155/ST398/ST58), D (n = 2/ST117/ST493), B2 (n = 1/ST127), and A (n = 1/ST746) phylogroups. Strain virulence scores varied from 3 to 12, and frequently harbored the pathogenicity island PAI IV536.The intestinal tract of healthy children constitute an important reservoir of ESBL producing E. coli. Thus, improvement of hygiene measures mainly in the school environment and rational use of antibiotics would be of great help in preventing selection and diffusion of resistant strains from intestinal microbiota.