The cys-loop ligand-gated ion channel gene family of Tetranychus urticae: Implications for acaricide toxicology and a novel mutation associated with abamectin resistance

  1. Dermauw, W. 5
  2. Ilias, A. 2
  3. Riga, M. 2
  4. Tsagkarakou, A. 3
  5. Grbić, M. 14
  6. Tirry, L. 5
  7. Van Leeuwen, T. 5
  8. Vontas, J. 2
  1. 1 University of Western Ontario
    info

    University of Western Ontario

    London, Canadá

    ROR https://ror.org/02grkyz14

  2. 2 University of Crete
    info

    University of Crete

    Heraklion, Grecia

    ROR https://ror.org/00dr28g20

  3. 3 Hellenic Agricultural Organization Demeter, Plant Protection Institute of Heraklion, Laboratory of Entomology and Agricultural Zoology, 71003 Heraklion, Greece
  4. 4 Instituto de Ciencias de la Vid y del Vino
    info

    Instituto de Ciencias de la Vid y del Vino

    Logroño, España

    ROR https://ror.org/01rm2sw78

  5. 5 Ghent University
    info

    Ghent University

    Gante, Bélgica

    ROR https://ror.org/00cv9y106

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Revista:
Insect Biochemistry and Molecular Biology

ISSN: 0965-1748

Año de publicación: 2012

Volumen: 42

Número: 7

Páginas: 455-465

Tipo: Artículo

DOI: 10.1016/J.IBMB.2012.03.002 PMID: 22465149 SCOPUS: 2-s2.0-84861462032 GOOGLE SCHOLAR

Otras publicaciones en: Insect Biochemistry and Molecular Biology

Repositorio institucional: lockAcceso abierto Editor

Resumen

The cys-loop ligand-gated ion channel (cysLGIC) super family of . Tetranychus urticae, the two-spotted spider mite, represents the largest arthropod cysLGIC super family described to date and the first characterised one within the group of chelicerates. Genome annotation, phylogenetic analysis and comparison of the cysLGIC subunits with their counterparts in insects reveals that the . T. urticae genome encodes for a high number of glutamate- and histamine-gated chloride channel genes (GluCl and HisCl) compared to insects. Three orthologues of the insect γ-aminobutyric acid (GABA)-gated chloride channel gene . Rdl were detected. Other cysLGIC groups, such as the nAChR subunits, are more conserved and have clear insect orthologues. Members of cysLGIC family mediate endogenous chemical neurotransmission and they are prime targets of insecticides. Implications for toxicology associated with the identity and specific features of . T. urticae family members are discussed. We further reveal the accumulation of known and novel mutations in different GluCl channel subunits (Tu_GluCl1 and Tu_GluCl3) associated with abamectin resistance in . T. urticae, and provide genetic evidence for their causality. Our study provides useful toxicological insights for the exploration of the . T. urticae cysLGIC subunits as putative molecular targets for current and future chemical control strategies. © 2012 Elsevier Ltd.