A horizontally transferred cyanase gene in the spider mite Tetranychus urticae is involved in cyanate metabolism and is differentially expressed upon host plant change

  1. Wybouw, N. 5
  2. Balabanidou, V. 1
  3. Ballhorn, D.J. 2
  4. Dermauw, W. 5
  5. Grbić, M. 34
  6. Vontas, J. 1
  7. Van Leeuwen, T. 5
  1. 1 University of Crete
    info

    University of Crete

    Heraklion, Grecia

    ROR https://ror.org/00dr28g20

  2. 2 Portland State University
    info

    Portland State University

    Portland, Estados Unidos

    ROR https://ror.org/00yn2fy02

  3. 3 University of Western Ontario
    info

    University of Western Ontario

    London, Canadá

    ROR https://ror.org/02grkyz14

  4. 4 Instituto de Ciencias de la Vid y del Vino
    info

    Instituto de Ciencias de la Vid y del Vino

    Logroño, España

    ROR https://ror.org/01rm2sw78

  5. 5 Ghent University
    info

    Ghent University

    Gante, Bélgica

    ROR https://ror.org/00cv9y106

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Revista:
Insect Biochemistry and Molecular Biology

ISSN: 0965-1748

Año de publicación: 2012

Volumen: 42

Número: 12

Páginas: 881-889

Tipo: Artículo

DOI: 10.1016/J.IBMB.2012.08.002 PMID: 22960016 SCOPUS: 2-s2.0-84868448167 GOOGLE SCHOLAR

Otras publicaciones en: Insect Biochemistry and Molecular Biology

Repositorio institucional: lockAcceso abierto Editor

Resumen

The genome of the phytophagous two-spotted spider mite Tetranychus urticae was recently sequenced, representing the first complete chelicerate genome, but also the first genome of a highly polyphagous agricultural pest. Genome analysis revealed the presence of an unexpected high number of cases of putative horizontal gene transfers, including a gene that encodes a cyanase or cyanate lyase. In this study we show by recombinant expression that the T. urticae cyanase remained functionally active after horizontal gene transfer and has a high affinity for cyanate. Cyanases were also detected in other plant parasitic spider mites species such as Tetranychus evansi and Panonychus citri, suggesting that an ancient gene transfer occurred before the diversification within the Tetranychidae family. To investigate the potential role of cyanase in the evolution of plant parasitic spider mites, we studied cyanase expression patterns in T. urticae in relation to host plant range and cyanogenesis, a common plant defense mechanism. Spider mites can alter cyanase expression levels after transfer to several new host plants, including the cyanogenic Phaseolus lunatus. However, the role of cyanase is probably not restricted to cyanide response, but likely to the plant nutritional quality as a whole. We finally discuss potential interactions between cyanase activity and pyrimidine and amino acid synthesis. © 2012 Elsevier Ltd.