High resolution genetic mapping uncovers chitin synthase-1 as the target-site ofthe structurally diverse mite growth inhibitors clofentezine, hexythiazox and etoxazole in Tetranychus urticae

  1. Demaeght, P. 4
  2. Osborne, E.J. 1
  3. Odman-Naresh, J. 3
  4. Grbić, M. 26
  5. Nauen, R. 7
  6. Merzendorfer, H. 3
  7. Clark, R.M. 1
  8. Van Leeuwen, T. 45
  1. 1 University of Utah
    info

    University of Utah

    Salt Lake City, Estados Unidos

    ROR https://ror.org/03r0ha626

  2. 2 University of Western Ontario
    info

    University of Western Ontario

    London, Canadá

    ROR https://ror.org/02grkyz14

  3. 3 University of Osnabrück
    info

    University of Osnabrück

    Osnabrück, Alemania

    ROR https://ror.org/04qmmjx98

  4. 4 Ghent University
    info

    Ghent University

    Gante, Bélgica

    ROR https://ror.org/00cv9y106

  5. 5 University of Amsterdam
    info

    University of Amsterdam

    Ámsterdam, Holanda

    ROR https://ror.org/04dkp9463

  6. 6 Instituto de Ciencias de la Vid y del Vino
    info

    Instituto de Ciencias de la Vid y del Vino

    Logroño, España

    ROR https://ror.org/01rm2sw78

  7. 7 R and D, Pest Control Biology, Bayer CropScience, Alfred Nobel Str. 50, D-40789 Monheim, Germany
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Revista:
Insect Biochemistry and Molecular Biology

ISSN: 0965-1748

Año de publicación: 2014

Volumen: 51

Número: 1

Páginas: 52-61

Tipo: Artículo

DOI: 10.1016/J.IBMB.2014.05.004 PMID: 24859419 SCOPUS: 2-s2.0-84902328055 GOOGLE SCHOLAR

Otras publicaciones en: Insect Biochemistry and Molecular Biology

Repositorio institucional: lockAcceso abierto Editor

Resumen

The acaricides clofentezine, hexythiazox and etoxazole are commonly referred to as 'mite growth inhibitors', and clofentezine and hexythiazox have been used successfully for the integrated control of plant mite pests for decades. Although they are still important today, their mode of action has remained elusive. Recently, a mutation in chitin synthase 1 (CHS1) was linked to etoxazole resistance. In this study, we identified and investigated a Tetranychus urticae strain (HexR) harboring recessive, monogenic resistance to each of hexythiazox, clofentezine, and etoxazole. To elucidate if there is a common genetic basis for the observed cross-resistance, we adapted a previously developed bulk segregant analysis method to map with high resolution a single, shared resistance locus for all three compounds. This finding indicates that the underlying molecular basis for resistance to all three compounds is identical. This locus is centered on the CHS1 gene, and as supported by additional genetic and biochemical studies, a non-synonymous variant (I1017F) in CHS1 associates with resistance to each of the tested acaricides in HexR. Our findings thus demonstrate a shared molecular mode of action for the chemically diverse mite growth inhibitors clofentezine, hexythiazox and etoxazole as inhibitors of an essential, non-catalytic activity of CHS1. Given the previously documented cross-resistance between clofentezine, hexythiazox and the benzyolphenylurea (BPU) compounds flufenoxuron and cycloxuron, CHS1 should be also considered as a potential target-site of insecticidal BPUs. © 2014 Elsevier Ltd.