Molecular characterization of extended-spectrum-beta-lactamase-producing Escherichia coli isolates from red foxes in Portugal

  1. Radhouani, H. 23
  2. Igrejas, G. 3
  3. Gonçalves, A. 23
  4. Estepa, V. 1
  5. Sargo, R. 3
  6. Torres, C. 1
  7. Poeta, P. 23
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Center of Studies of Animal and Veterinary Sciences, Vila Real, Portugal
  3. 3 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

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Revista:
Archives of Microbiology

ISSN: 0302-8933

Año de publicación: 2013

Volumen: 195

Número: 2

Páginas: 141-144

Tipo: Artículo

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DOI: 10.1007/S00203-012-0853-7 SCOPUS: 2-s2.0-84873734927 WoS: WOS:000314270900007 GOOGLE SCHOLAR

Otras publicaciones en: Archives of Microbiology

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2013/00001/001

Resumen

The presence of broad-spectrum-cephalosporin-resistant Escherichia coli isolates and the implicated mechanisms of resistance and virulence factor genes were investigated in red fox (Vulpes vulpes) in Portugal. Cefotaxime-resistant E. coli isolates were isolated from two of 52 fecal samples (4 %), being both ESBL producers. The β-lactamase genes found in the two isolates were bla SHV-12 + bla TEM-1b. The tet(A) and sul2 genes were also detected in these isolates, together with the non-classical class 1 integron (intI1-dfrA12-orfF-aadA2-cmlA1-aadA1-qacH-IS440-sul3) with the PcH1 promoter. The two isolates belonged to the phylogroup A. Amino acid changes in GyrA (S83L + D87G) and ParC (S80I) proteins were identified in our study. Concerning MLST typing, both isolates were assigned to ST1086, never found before in wild animals, and they presented closely related PFGE patterns. This study reveals the presence of ESBL-producing E. coli isolates, in a wild ecosystem, which could be disseminated through the environment to other niches. © 2012 Springer-Verlag Berlin Heidelberg.