Proteomic evaluation of escherichia coli isolates from human clinical strains

  1. Pinto, L. 2222
  2. Poeta, P. 22
  3. Radhouani, H. 2222
  4. Coelho, C. 2222
  5. Carvalho, C. 22
  6. Rodrigues, J. 22
  7. Torres, C. 1
  8. Vitorino, R. 3
  9. Domingues, P. 3
  10. Igrejas, G. 22
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

  3. 3 Universidade de Aveiro
    info

    Universidade de Aveiro

    Aveiro, Portugal

    ROR https://ror.org/00nt41z93

Mostrar afiliaciones +
Revista:
Journal of Integrated OMICS

ISSN: 2182-0287

Año de publicación: 2011

Volumen: 1

Número: 1

Páginas: 42-48

Tipo: Artículo

beta Ver similares en nube de resultados
DOI: 10.5584/JIOMICS.V1I1.20 SCOPUS: 2-s2.0-85038583633 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Integrated OMICS

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

Acquired resistance to beta-lactams is mainly mediated by extended-spectrum beta-lactamases (ESBLs) that confer bacterial resistance to all beta-lactams except carbapenems and cephamycins, which are inhibited by other beta-lactamase inhibitors such as clavulanic acid. Although ESBLs still constitute the first cause of resistance to beta-lactams among Escherichia coli, other “new beta-lactamases” conferring resistance to carbapenems, such as metallo-beta-lactamases (MBL) and KPC carbapenemases, or to cephamycins, such as CMY enzymes, have more recently emerged and are often associated with ESBLs. In order to identify and characterize the proteome of extended-spectrum β-lactamase (ESBL) type TEM-52 and CMY-2 producing-Escherichia coli strains of human clinical origin a bidimensional electrophoresis (2-DE) technique with an isoelectric focusing followed by a SDS-PAGE, were used. Full proteomic studies were conducted in the same IEF and SDS-PAGE conditions, for two protein samples of E. coli strains with similar antibiotic-resistance profiles recovered from human clinical sources. A total of 64 and 91 spots were recovered and identified in C583 and C580 strains, respectively. Our results will be helpful for further understanding of antibiotic-resistant mechanism. © 2011, Proteomass Scientific Society. All rights reserved.