Proteomic study in an Escherichia coli strain from seagulls of the berlengas natural reserve of Portugal

  1. Pinto, L. 2222
  2. Poeta, P. 22
  3. Radhouani, H. 2222
  4. Coelho, C. 2222
  5. Carvalho, C. 22
  6. Rodrigues, J. 22
  7. Torres, C. 1
  8. Vitorino, R. 3
  9. Domingues, P. 3
  10. Igrejas, G. 22
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

  3. 3 Universidade de Aveiro
    info

    Universidade de Aveiro

    Aveiro, Portugal

    ROR https://ror.org/00nt41z93

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Revista:
Journal of Integrated OMICS

ISSN: 2182-0287

Año de publicación: 2011

Volumen: 1

Número: 1

Páginas: 36-41

Tipo: Artículo

DOI: 10.5584/JIOMICS.V1I1.19 SCOPUS: 2-s2.0-84982279199 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Integrated OMICS

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

The increasing bacterial resistance among common pathogens is threatening the effectiveness of several antibiotics. This represents a serious public health problem as such bacterial strains have already been detected in domestic, wild-life animals and humans. Using Escherichia coli as a model organism, we applied a proteomic approach to the topic of antimicrobial resistance. In order to identify and characterize the proteome of extendedspectrum β-lactamase (ESBL) type TEM-52 producing-Escherichia coli strain of a faecal sample taken from Yellow-legged seagulls (Larus cachinnans) a bidimensional electrophoresis (2-DE) technique with an isoelectric focusing followed by a SDS-PAGE, was used. Eighty seven individualized protein spots were identified. All were suitable for peptide mass fingerprinting by a mass spectrometric technique (MALDI/TOF MS). Their identification was carried out by searching appropriate bioinformatic databases. All proteins were related to E. coli strains. Detection of proteins related to several E. coli strains linked with virulent and enterohaemorrhagic consequences in ESBL producing-E. coli isolates of seagull samples raises the question of how such similarities arise bearing in mind these remarkably different microbial ecosystems. © 2011, Proteomass Scientific Society. All rights reserved.