A genetic linkage map of cowpea (Vigna unguiculata) developed from a cross between two inbred, domesticated lines

  1. Menéndez, C.M. 13
  2. Hall, A.E. 2
  3. Gepts, P. 1
  1. 1 University of California, Davis
    info

    University of California, Davis

    Davis, Estados Unidos

    ROR https://ror.org/05rrcem69

  2. 2 University of California, Riverside
    info

    University of California, Riverside

    Riverside, Estados Unidos

    ROR https://ror.org/03nawhv43

  3. 3 MP1Z, Carl-von-Luné-Weg 10, 50829, Köln, Germany
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Revista:
Theoretical and Applied Genetics

ISSN: 0040-5752

Año de publicación: 1997

Volumen: 95

Número: 8

Páginas: 1210-1217

Tipo: Artículo

DOI: 10.1007/S001220050683 SCOPUS: 2-s2.0-0031445141 GOOGLE SCHOLAR

Otras publicaciones en: Theoretical and Applied Genetics

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

We have constructed a genetic linkage map within the cultivated gene pool of cowpea (2n = 2x = 22) from an F8 recombinant inbred population (94 individuals) derived from a cross between the inbreds IT84S-2049 and 524B. These breeding lines, developed in Nigeria and California, show contrasting reactions against several pests and diseases and differ in several morphological traits. Parental lines were screened with 332 random RAPD decamers, 74 RFLP probes (bean, cowpea and mung bean genomic DNA clones), and 17 AFLP primer combinations. RAPD primers were twice as efficient as AFLP primers and RFLP probes in detecting polymorphisms in this cross. The map consists of 181 loci, comprising 133 RAPDs, 19 RFLPs, 25 AFLPs, three morphological/classical markers, and a biochemical marker (dehydrin). These markers identified 12 linkage groups spanning 972 cM with an average distance of 6.4 cM between markers. Linkage groups ranged from 3 to 257 cM in length and included from 2 to 41 markers, respectively. A gene for earliness was mapped on linkage group 2. Seed weight showed a significant association with a RAPD marker on linkage group 5. This map should facilitate the identification of markers that 'tag' genes for pest and disease resistance and other traits in the cultivated gene pool of cowpea.