The salivary protein repertoire of the polyphagous spider mite tetranychus urticae: A quest for effectors

  1. Jonckheere, W. 56
  2. Dermauw, W. 6
  3. Zhurov, V. 4
  4. Wybouw, N. 5
  5. Van Den Bulcke, J. 6
  6. Villarroel, C.A. 5
  7. Greenhalgh, R. 3
  8. Grbić, M. 410
  9. Schuurink, R.C. 5
  10. Tirry, L. 6
  11. Baggerman, G. 2
  12. Clark, R.M. 3
  13. Kant, M.R. 5
  14. Vanholme, B. 8
  15. Menschaert, G. 6
  16. Van Leeuwen, T. 56
  1. 1 Center for Cell and Genome Science, University of Utah, Salt Lake City 257 South 1400, East Utah, United States
  2. 2 University of Antwerp
    info

    University of Antwerp

    Amberes, Bélgica

    ROR https://ror.org/008x57b05

  3. 3 University of Utah
    info

    University of Utah

    Salt Lake City, Estados Unidos

    ROR https://ror.org/03r0ha626

  4. 4 University of Western Ontario
    info

    University of Western Ontario

    London, Canadá

    ROR https://ror.org/02grkyz14

  5. 5 University of Amsterdam
    info

    University of Amsterdam

    Ámsterdam, Holanda

    ROR https://ror.org/04dkp9463

  6. 6 Ghent University
    info

    Ghent University

    Gante, Bélgica

    ROR https://ror.org/00cv9y106

  7. 7 Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium
  8. 8 Department of Plant Systems Biology, VIB, Gent, Belgium
  9. 9 Flemish Institute for Technological Research (VITO), Boeretang 200, Mol, Belgium
  10. 10 Instituto de Ciencias de la Vid y del Vino
    info

    Instituto de Ciencias de la Vid y del Vino

    Logroño, España

    ROR https://ror.org/01rm2sw78

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Revista:
Molecular and Cellular Proteomics

ISSN: 1535-9476

Año de publicación: 2016

Volumen: 15

Número: 12

Páginas: 3594-3613

Tipo: Artículo

DOI: 10.1074/MCP.M116.058081 PMID: 27703040 SCOPUS: 2-s2.0-85002496956 GOOGLE SCHOLAR

Otras publicaciones en: Molecular and Cellular Proteomics

Resumen

The two-spotted spider mite Tetranychus urticae is an extremely polyphagous crop pest. Alongside an unparalleled detoxification potential for plant secondary metabolites, it has recently been shown that spider mites can attenuate or even suppress plant defenses. Salivary constituents, notably effectors, have been proposed to play an important role in manipulating plant defenses and might determine the outcome of plant-mite interactions. Here, the proteomic composition of saliva from T. urticae lines adapted to various host plants-bean, maize, soy, and tomato-was analyzed using a custom-developed feeding assay coupled with nano-LC tandem mass spectrometry. About 90 putative T. urticae salivary proteins were identified. Many are of unknown function, and in numerous cases belonging to multimembered gene families. RNAseq expression analysis revealed that many genes coding for these salivary proteins were highly expressed in the proterosoma, the mite body region that includes the salivary glands. A subset of genes encoding putative salivary proteins was selected for whole-mount in situ hybridization, and were found to be expressed in the anterior and dorsal podocephalic glands. Strikingly, host plant dependent expression was evident for putative salivary proteins, and was further studied in detail by micro-array based genome-wide expression profiling. This meta-analysis revealed for the first time the salivary protein repertoire of a phytophagous chelicerate. The availability of this salivary proteome will assist in unraveling the molecular interface between phytophagous mites and their host plants, and may ultimately facilitate the development of mite-resistant crops. Furthermore, the technique used in this study is a time- and resourceefficient method to examine the salivary protein composition of other small arthropods for which saliva or salivary glands cannot be isolated easily. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.