Development of a standard set of microsatellite reference alleles for identification of grape cultivars

  1. This, P. 9
  2. Jung, A. 5
  3. Boccacci, P. 1
  4. Borrego, J. 7
  5. Botta, R. 1
  6. Costantini, L. 10
  7. Crespan, M. 11
  8. Dangl, G.S. 4
  9. Eisenheld, C. 2
  10. Ferreira-Monteiro, F. 8
  11. Grando, S. 10
  12. Ibáñez, J. 7
  13. Lacombe, T. 9
  14. Laucou, V. 9
  15. Magalhães, R. 6
  16. Meredith, C.P. 4
  17. Milani, N. 11
  18. Peterlunger, E. 3
  19. Regner, F. 2
  20. Zulini, L. 3
  21. Maul, E. 5
  1. 1 University of Turin
    info

    University of Turin

    Turín, Italia

    ROR https://ror.org/048tbm396

  2. 2 Department for Grapevine Breeding, HBLA u. BA Klosterneuburg, 2103 Langenzersdorf, Rehgraben 2, Austria
  3. 3 Università di Udine
    info

    Università di Udine

    Udine, Italia

    ROR https://ror.org/05ht0mh31

  4. 4 University of California, Davis
    info

    University of California, Davis

    Davis, Estados Unidos

    ROR https://ror.org/05rrcem69

  5. 5 Julius Kühn-Institut
    info

    Julius Kühn-Institut

    Quedlinburg, Alemania

    ROR https://ror.org/022d5qt08

  6. 6 Inst. Cie. Biomed. de Abel Salazar, Universidade do Porto, Largo Prof. Abel Salazar, 2, 4099-003 Porto, Portugal
  7. 7 Instituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario
    info

    Instituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario

    Madrid, España

  8. 8 Inst. Portugues Viticultura Enologia, Rua Eng. Frederico Ulrich 2650, 4470-605 Maia, Portugal
  9. 9 Institut National de la Recherche Agronomique
    info

    Institut National de la Recherche Agronomique

    París, Francia

  10. 10 Laboratory of Molecular Genetics, Ist. Agrario S. Michele All' Adige, Via Mach, 38010 Trento, Italy
  11. 11 Sezione Ampelografia M., Ist. Sperimentale per la Viticoltura, via Casoni 13/A, 31058 Susegana (Treviso), Italy
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Revista:
Theoretical and Applied Genetics

ISSN: 0040-5752

Año de publicación: 2004

Volumen: 109

Número: 7

Páginas: 1448-1458

Tipo: Artículo

DOI: 10.1007/S00122-004-1760-3 PMID: 15565426 SCOPUS: 2-s2.0-19944427794 GOOGLE SCHOLAR

Otras publicaciones en: Theoretical and Applied Genetics

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

In order to investigate the comparability of microsatellite profiles obtained in different laboratories, ten partners in seven countries analyzed 46 grape cultivars at six loci (VVMD5, VVMD7, VVMD27, VVS2, VrZAG62, and VrZAG79). No effort was made to standardize equipment or protocols. Although some partners obtained very similar results, in other cases different absolute allele sizes and, sometimes, different relative allele sizes were obtained. A strategy for data comparison by means of reference to the alleles detected in well-known cultivars was proposed. For each marker, each allele was designated by a code based on the name of the reference cultivar carrying that allele. Thirty-three cultivars, representing from 13 to 23 alleles per marker, were chosen as references. After the raw data obtained by the different partners were coded, more than 97% of the data were in agreement. Minor discrepancies were attributed to errors, suboptimal amplification and visualization, and misscoring of heterozygous versus homozygous allele pairs. We have shown that coded microsatellite data produced in different laboratories with different protocols and conditions can be compared, and that it is suitable for the identification and SSR allele characterization of cultivars. It is proposed that the six markers employed here, already widely used, be adopted as a minimal standard marker set for future grapevine cultivar analyses, and that additional cultivars be characterized by means of the coded reference alleles presented here. The complete database is available at http://www.genres.de/eccdb/vitis/. Cuttings of the 33 reference cultivars are available on request from the Institut National de la Recherche Agronomique Vassal collection (didier.vares@ensam.inra.fr). © Springer-Verlag 2004.