Effect of the diet type and temperature on the C. elegans transcriptome

  1. Gómez-Orte, E. 2
  2. Cornes, E. 15
  3. Zheleva, A. 2
  4. Sáenz-Narciso, B. 2
  5. de Toro, M. 2
  6. Iñiguez, M. 2
  7. López, R. 2
  8. San-Juan, J.-F. 6
  9. Ezcurra, B. 2
  10. Sacristán, B. 4
  11. Sánchez-Blanco, Adolfo 3
  12. Cerón, J. 1
  13. Cabello, J. 2
  1. 1 Institut d'Investigació Biomédica de Bellvitge
    info

    Institut d'Investigació Biomédica de Bellvitge

    Barcelona, España

    ROR https://ror.org/0008xqs48

  2. 2 Centro de Investigación Biomédica de La Rioja
    info

    Centro de Investigación Biomédica de La Rioja

    Logroño, España

    ROR https://ror.org/03vfjzd38

  3. 3 University of Hartford
    info

    University of Hartford

    West Hartford, Estados Unidos

    ROR https://ror.org/034gcgd08

  4. 4 Hospital San Pedro
    info

    Hospital San Pedro

    Logroño, España

    ROR https://ror.org/031va0421

  5. 5 Institut Pasteur, Paris, France
  6. 6 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

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Zeitschrift:
Oncotarget

ISSN: 1949-2553

Datum der Publikation: 2018

Ausgabe: 9

Nummer: 11

Seiten: 9556-9571

Art: Artikel

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DOI: 10.18632/ONCOTARGET.23563 SCOPUS: 2-s2.0-85041717291 GOOGLE SCHOLAR

Andere Publikationen in: Oncotarget

Institutionelles Repository: lock_openOpen Access Editor

Zusammenfassung

The transcriptomes of model organisms have been defined under specific laboratory growth conditions. The standard protocol for Caenorhabditis elegans growth and maintenance is 20°C on an Escherichia coli diet. Temperatures ranging from 15°C to 25°C or feeding with other species of bacteria are considered physiological conditions, but the effect of these conditions on the worm transcriptome has not been well characterized. Here, we compare the global gene expression profile for the reference Caenorhabditis elegans strain (N2) grown at 15°C, 20°C, and 25°C on two different diets, Escherichia coli and Bacillus subtilis. When C. elegans were fed E. coli and the growth temperature was increased, we observed an enhancement of defense response pathways and down-regulation of genes associated with metabolic functions. However, when C. elegans were fed B. subtilis and the growth temperature was increased, the nematodes exhibited a decrease in defense response pathways and an enhancement of expression of genes associated with metabolic functions. Our results show that C. elegans undergo significant metabolic and defense response changes when the maintenance temperature fluctuates within the physiological range and that the degree of pathogenicity of the bacterial diet can further alter the worm transcriptome. © Gómez-Orte et al.