Emergence of plasmid-mediated colistin-resistance in CMY-2-producing Escherichia coli of lineage ST2197 in a Tunisian poultry farm

  1. Maamar, E. 3
  2. Alonso, C.A. 2
  3. Hamzaoui, Z. 3
  4. Dakhli, N. 3
  5. Abbassi, M.S. 3
  6. Ferjani, S. 3
  7. Saidani, M. 13
  8. Boutiba-Ben Boubaker, I. 3
  9. Torres, C. 2
  1. 1 Hôpital Charles-Nicolle
    info

    Hôpital Charles-Nicolle

    Túnez, Túnez

    ROR https://ror.org/02f6ghw27

  2. 2 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  3. 3 Université de Tunis El Manar
    info

    Université de Tunis El Manar

    Túnez, Túnez

    ROR https://ror.org/029cgt552

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Revista:
International Journal of Food Microbiology

ISSN: 0168-1605

Any de publicació: 2018

Volum: 269

Pàgines: 60-63

Tipus: Article

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DOI: 10.1016/J.IJFOODMICRO.2018.01.017 SCOPUS: 2-s2.0-85041388110 WoS: WOS:000428103700008 GOOGLE SCHOLAR

Altres publicacions en: International Journal of Food Microbiology

Objectius de Desenvolupament Sostenible

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Resum

Our study aimed to investigate colistin resistance and the mechanisms involved in a collection of 35 extended-spectrum beta-lactamase (ESBL) and 13 CMY-2-producing E. coli strains which were previously recovered from chicken gut microbiota in Tunisia, as well as to determine the genetic location of mcr genes. Forty-eight ESBL and CMY-2-producing E. coli strains were obtained from 137 fecal samples of healthy chickens during 2013. These strains were tested for colistin resistance by the broth microdilution method, and screened for mcr-1 and mcr-2 genes by PCR. Two of these strains were colistin-resistant (MIC = 8 mg/L). Both harbored the mcr-1 gene, were CMY-2 producers, and were additionally resistant to tetracycline, ciprofloxacin, chloramphenicol, gentamicin, tobramycin and trimethoprim-sulfamethoxazole. They shared phylogroup A, the same pulsed-field gel electrophoresis (PFGE)-pattern, and were typed as ST2197. In both strains, ISApl1 and pap2 were detected upstream and downstream of mcr-1 gene, respectively. The analysis of the two mcr-1-positive strains and their transconjugants by PCR-based replicon typing and S1-PFGE, demonstrated that mcr-1 gene is linked to an IncP plasmid (~242 kb), and blaCMY-2 to an IncI1 plasmid (97 kb). The occurrence of E. coli harboring mcr-1 gene among intestinal microbiota in poultry and its location on a conjugative plasmid could represent a risk for public health. The evolution of this type of resistant microorganisms should be evaluated in the future