Iberian wolf as a reservoir of extended-spectrum β-lactamase-producing Escherichia coli of the TEM, SHV, and CTX-M groups

  1. Gonçalves, A. 23
  2. Igrejas, G. 3
  3. Radhouani, H. 23
  4. Estepa, V. 1
  5. Pacheco, R. 23
  6. Monteiro, R. 23
  7. Brito, F. 23
  8. Guerra, A. 4
  9. Petrucci-Fonseca, F. 4
  10. Torres, C. 1
  11. Poeta, P. 23
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Center for Animal Science and Veterinary, Vila Real, Portugal
  3. 3 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

  4. 4 Universidade de Lisboa
    info

    Universidade de Lisboa

    Lisboa, Portugal

    ROR https://ror.org/01c27hj86

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Revista:
Microbial Drug Resistance

ISSN: 1076-6294

Año de publicación: 2012

Volumen: 18

Número: 2

Páginas: 215-219

Tipo: Artículo

DOI: 10.1089/MDR.2011.0145 PMID: 22185366 SCOPUS: 2-s2.0-84859559869 WoS: WOS:000302590600017 GOOGLE SCHOLAR

Otras publicaciones en: Microbial Drug Resistance

Resumen

The intensive use of antibiotics in human and veterinary medicine, associated with mechanisms of bacterial genetic transfer, caused a selective pressure that contributed to the dissemination of antimicrobial resistance in different bacteria groups and throughout different ecosystems. Iberian wolf, due to his predatory and wild nature, may serve as an important indicator of environmental contamination with antimicrobial resistant bacteria. The aim of this study was to characterize the diversity of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates within the fecal microbiota of Iberian wolf. Additionally, the identification of other associated resistance genes, phylogenetic groups, and the detection of virulence determinants were also focused on in this study. From 2008 to 2009, 237 fecal samples from Iberian wolf were collected in Portugal. E. coli isolates with TEM-52, SHV-12, CTX-M-1, and CTX-M-14-type ESBLs were detected in 13 of these samples (5.5%). This study reveals the presence of ESBL-producing E. coli isolates, in a wild ecosystem, which could be disseminated through the environment. Moreover, the presence of resistant genes in integrons and the existence of virulence determinants were shown. The association between antibiotic resistance and virulence determinants should be monitored, as it constitutes a serious public health problem. © 2012 Mary Ann Liebert, Inc.