Acquisition of carbapenem resistance in multiresistant Klebsiella pneumoniae strains harbouring bla CTX-M-15, qnrS1 and aac(6')-Ib-cr genes

  1. Ruiz, E. 1
  2. Ocampo-Sosa, A.A. 4
  3. Rezusta, A. 3
  4. Revillo, M.J. 3
  5. Román, E. 4
  6. Torres, C. 1
  7. Martínez-Martínez, L. 24
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Universidad de Cantabria
    info

    Universidad de Cantabria

    Santander, España

    ROR https://ror.org/046ffzj20

  3. 3 Hospital Miguel Servet
    info

    Hospital Miguel Servet

    Zaragoza, España

    ROR https://ror.org/01r13mt55

  4. 4 Hospital Universitario Marqués de Valdecilla
    info

    Hospital Universitario Marqués de Valdecilla

    Santander, España

    ROR https://ror.org/01w4yqf75

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Journal:
Journal of Medical Microbiology

ISSN: 0022-2615

Year of publication: 2012

Volume: 61

Issue: 5

Pages: 672-677

Type: Article

DOI: 10.1099/JMM.0.038083-0 PMID: 22282462 SCOPUS: 2-s2.0-84859815887 WoS: WOS:000304217700009 GOOGLE SCHOLAR

More publications in: Journal of Medical Microbiology

Institutional repository: lock_openOpen access Editor

Abstract

Three closely related Klebsiella pneumoniae strains isolated from the same patient harboured bla CTX-M-15, bla OXA-1, bla SHV-11, qnrS1, aac(69)-Ib-cr, oqxAB, aac(3)-II and aph(39)-Ia genes. Two of the isolates were recovered after treatment with meropenem and showed resistance to carbapenems. Sequencing of ompK35 and ompK36 porin genes of the carbapenem-resistant strains revealed the presence of premature stop codons in both, and OmpK35 and OmpK36 porins were not detected by SDS-PAGE. One carbepenem-resistant strain showed a high amount of LamB protein and did not express OmpK26 porin whereas the other strain expressed OmpK26 but not LamB. The lack of major porins apparently causes changes in the expression of other, specific, porins. © 2012 SGM.